Anti-VPS35/MEM3 antibody (C-Term) (STJ70506)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-VPS35/MEM3 (C-Term) is suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Applications | Pep-ELISA/WB/IHC/IF |
| Host | Goat |
| Reactivity | Human/Mouse/Rat/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 90kDa band observed in Human (Cerebellum) , Mouse and Rat Brain lysates and in lysates of cell line HepG2, and approx. 85kDa observed in lysates of cell line HEK293 (calculated |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | VPS35 |
| Gene ID | 55737 |
| Uniprot ID | VPS35_HUMAN |
| Accession Number | NP_060676.2 |
| Immunogen Region | C-Term |
| Immunogen Sequence | SPESEGPIYEGLIL |
| Specificity | Note there is a hypothetical protein called similar to vacuolar protein sorting 35 (XP_040192.1) , which is virtually identical. |
Additional Info
| Function | Acts as a component of the retromer cargo-selective complex (CSC). The CSC is believed to be the core functional component of retromer or respective retromer complex variants acting to prevent missorting of selected transmembrane cargo proteins into the lysosomal degradation pathway. The recruitment of the CSC to the endosomal membrane involves RAB7A and SNX3. The CSC seems to associate with the cytoplasmic domain of cargo proteins predominantly via VPS35.however, these interactions seem to be of low affinity and retromer SNX proteins may also contribute to cargo selectivity thus questioning the classical function of the CSC. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX3-retromer mediates the retrograde endosome-to-TGN transport of WLS distinct from the SNX-BAR retromer pathway. The SNX27-retromer is believed to be involved in endosome-to-plasma membrane trafficking and recycling of a broad spectrum of cargo proteins. The CSC seems to act as recruitment hub for other proteins, such as the WASH complex and TBC1D5 (Probable). Required for retrograde transport of lysosomal enzyme receptor IGF2R and SLC11A2. Required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). Required for endosomal localization of WASHC2C. Mediates the association of the CSC with the WASH complex via WASHC2. Required for the endosomal localization of TBC1D5. (Microbial infection) The heterotrimeric retromer cargo-selective complex (CSC) mediates the exit of human papillomavirus from the early endosome and the delivery to the Golgi apparatus. |
| Protein Name | Vacuolar Protein Sorting-Associated Protein 35Hvps35Maternal-Embryonic 3Vesicle Protein Sorting 35 |
| Database Links | Reactome: R-HSA-3238698 |
| Cellular Localisation | CytoplasmMembranePeripheral Membrane ProteinEndosomeEarly EndosomeLate EndosomeLocalizes To Tubular Profiles Adjacent To Endosomes |
| Alternative Antibody Names | Anti-Vacuolar Protein Sorting-Associated Protein 35 antibodyAnti-Hvps35 antibodyAnti-Maternal-Embryonic 3 antibodyAnti-Vesicle Protein Sorting 35 antibodyAnti-VPS35 antibodyAnti-MEM3 antibodyAnti-TCCCTA00141 antibody |
Information sourced from Uniprot.org