| Host: | Rabbit |
| Applications: | WB |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Vacuolar protein sorting-associated protein 33B (12-62 aa) is suitable for use in Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-2000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | VPS33B |
| Gene ID: | 26276 |
| Uniprot ID: | VP33B_HUMAN |
| Immunogen Region: | 12-62 aa |
| Specificity: | This antibody detects endogenous levels of VP33B at Human/Mouse/Rat |
| Immunogen: | Synthesized peptide derived from the human VP33B at the amino acid range 12-62 |
| Post Translational Modifications | Phosphorylated on tyrosine residues. (Microbial infection) Dephosphorylated by M.tuberculosis PtpA, which induces the reduction of host phagolysosome fusion in M.tuberculosis-infected macrophages. |
| Function | May play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes. Required for proper trafficking and targeting of the collagen-modifying enzyme lysyl hydroxylase 3 (LH3) to intracellular collagen. Mediates phagolysosomal fusion in macrophages. Proposed to be involved in endosomal maturation implicating VIPAS39. In epithelial cells, the VPS33B:VIPAS39 complex may play a role in the apical recycling pathway and in the maintenance of the apical-basolateral polarity. Seems to be involved in the sorting of specific cargos from the trans-Golgi network to alpha-granule-destined multivesicular bodies (MVBs) promoting MVBs maturation in megakaryocytes. |
| Protein Name | Vacuolar Protein Sorting-Associated Protein 33b Hvps33b |
| Database Links | Reactome: R-HSA-9636383 Reactome: R-HSA-9754560 |
| Cellular Localisation | Late Endosome Membrane Peripheral Membrane Protein Cytoplasmic Side Lysosome Membrane Early Endosome Cytoplasmic Vesicle Clathrin-Coated Vesicle Recycling Endosome Colocalizes In Clusters With Vipas39 At Cytoplasmic Organelles Colocalizes With Rab11a And Vipas39 On Recycling Endosomes Colocalizes With Ap-3 Clathrin Rab5 And Rab7b Colocalizes With M Tuberculosis Ptpa In The Cytosol Of Tuberculosis-Infected Macrophages And Associates With Phagosomes |
| Alternative Antibody Names | Anti-Vacuolar Protein Sorting-Associated Protein 33b antibody Anti-Hvps33b antibody Anti-VPS33B antibody |
Information sourced from Uniprot.org