• U2OS whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Vimentin (ABT281) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: U2OSPredicted band size: 54kDaObserved band size: 54kDa
  • Human endometrial adenocarcinoma tissue was stained with Anti-Vimentin (ABT281) Antibody
  • Human hepatocellular carcinoma tissue was stained with Anti-Vimentin (ABT281) Antibody
  • Human rectal carcinoma tissue was stained with Anti-Vimentin (ABT281) Antibody
  • Fluorescence multiplex immunohistochemical analysis of Human tonsil tissue (formalin-fixed paraffin-embedded section). Merged staining of Anti-Vimentin (STJ197198), Anti-p120 (STJ190083), Anti-Topoisomerase IIα (STJ197194). The immunostaining was performed on a Leica Biosystems BOND® MAX instrument with an Sextuple-Fluorescence kit. The section was incubated in 3 rounds of staining; sequentially for Anti-Vimentin (STJ197198 1:200), Anti-p120 (STJ190083 1:200), Anti-Topoisomerase IIα (STJ197194 1:200). ; each using a separate fluorescent tyramide signal amplification system. EDTA based antigen retrieval (Leica Biosystems BOND® Epitope Retrieval Solution 2, pH 9.0, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. DAPI (dark blue) was used as a nuclear counter stain. Microscopy and pseudocoloring of individual dyes was performed using a Slideviewer Imaging System (3D histech).
  • Fluorescence multiplex immunohistochemical analysis of Human tonsil tissue (formalin-fixed paraffin-embedded section). Merged staining of Anti-Vimentin (STJ197198), Anti-p120 (STJ190083), Anti-Topoisomerase IIα (STJ197194). The immunostaining was performed on a Leica Biosystems BOND® MAX instrument with an Sextuple-Fluorescence kit. The section was incubated in 3 rounds of staining; sequentially for Anti-Vimentin (STJ197198 1:200), Anti-p120 (STJ190083 1:200), Anti-Topoisomerase IIα (STJ197194 1:200). ; each using a separate fluorescent tyramide signal amplification system. EDTA based antigen retrieval (Leica Biosystems BOND® Epitope Retrieval Solution 2, pH 9.0, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. DAPI (dark blue) was used as a nuclear counter stain. Microscopy and pseudocoloring of individual dyes was performed using a Slideviewer Imaging System (3D histech).

Anti-VIM antibody (400-466 aa) [ABT281] (STJ197198)

SKU:
STJ197198

$226.80 - $305.10
Current Stock:
Host: Mouse
Applications: IHC/IF/WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Vimentin (400-466 aa) is suitable for use in Immunohistochemistry, Immunofluorescence and Western Blot research applications.
Clonality: Monoclonal
Clone ID: ABT281
Conjugation: Unconjugated
Isotype: IgG1k
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Dilution Range: IHC-P 1:100-500
WB 1:200-1000
IF 1:100-500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: VIM
Gene ID: 7431
Uniprot ID: VIME_HUMAN
Immunogen Region: 400-466 aa
Specificity: The antibody can specifically recognize human Vimentin protein. In western blotting of Hela cell lysate, the antibody can label a 54KDa band corresponding to Vimentin.
Immunogen: Synthesized peptide derived from the human Vimentin at the amino acid range 400-466
Post Translational Modifications Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin. One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33. Phosphorylated on tyrosine residues by SRMS. O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status. S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
Function Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
Protein Name Vimentin
Database Links Reactome: R-HSA-264870
Reactome: R-HSA-390522
Reactome: R-HSA-6785807
Reactome: R-HSA-9013422
Reactome: R-HSA-9613829
Reactome: R-HSA-9615710
Reactome: R-HSA-9646399
Cellular Localisation Cytoplasm
Cytoskeleton
Nucleus Matrix
Cell Membrane
Alternative Antibody Names Anti-Vimentin antibody
Anti-VIM antibody

Information sourced from Uniprot.org

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