• Human appendix tissue was stained with Anti-Vimentin (ABT019) Antibody
  • Human colon carcinoma tissue was stained with Anti-Vimentin (ABT019) Antibody
  • Human esophageal squamous cell carcinoma tissue was stained with Anti-Vimentin (ABT019) Antibody
  • Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Vimentin and anti-GAPDH antibody. The HRP-conjugated anti- mouse IgG antibody was used to detect the antibody. Lane 1: Wide type HEK293 cell lysate Lane 2: Vimentin knockout HEK293 cell lysatePredicted band size: 54kDaObserved band size: 54kDa

Anti-VIM antibody (400-466 aa) [ABT019] (STJ197249)

SKU:
STJ197249

£168.00 - £226.00
Current Stock:
Host: Mouse
Applications: IHC/WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Vimentin (400-466 aa) is suitable for use in Immunohistochemistry and Western Blot research applications.
Clonality: Monoclonal
Clone ID: ABT019
Conjugation: Unconjugated
Isotype: IgG2bk
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Dilution Range: IHC-P 1:100-500
WB 1:200-1000
IF 1:100-500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: VIM
Gene ID: 7431
Uniprot ID: VIME_HUMAN
Immunogen Region: 400-466 aa
Specificity: The antibody can specifically recognize human Vimentin protein. In western blotting of HEK293 cell lysate, the antibody can label a 54KDa band corresponding to Vimentin.
Immunogen: Synthesized peptide derived from the human Vimentin at the amino acid range 400-466
Post Translational Modifications Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin. One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33. Phosphorylated on tyrosine residues by SRMS. O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status. S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
Function Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
Protein Name Vimentin
Database Links Reactome: R-HSA-264870
Reactome: R-HSA-390522
Reactome: R-HSA-6785807
Reactome: R-HSA-9013422
Reactome: R-HSA-9613829
Reactome: R-HSA-9615710
Reactome: R-HSA-9646399
Cellular Localisation Cytoplasm
Cytoskeleton
Nucleus Matrix
Cell Membrane
Alternative Antibody Names Anti-Vimentin antibody
Anti-VIM antibody

Information sourced from Uniprot.org

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