| Host: | Goat |
| Applications: | Pep-ELISA/WB/IHC |
| Reactivity: | Human/Mouse/Rat/Dog |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-VHL (Internal) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:128000. WB: Approx 17kDa band observed in Human Ovary and Testis lysates and also in lysates of Mouse and Rat Testis and Brain and of all human and mouse cell lines tested (calculated MW of 19.7k |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | VHL |
| Gene ID: | 7428 |
| Uniprot ID: | VHL_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_000542.1; NP_937799.1 |
| Specificity: | This antibody is expected to recognise both reported isoforms (NP_000542.1 and NP_937799.1). |
| Immunogen Sequence: | RSLVKPENYRRLD |
| Function | Involved in the ubiquitination and subsequent proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Seems to act as a target recruitment subunit in the E3 ubiquitin ligase complex and recruits hydroxylated hypoxia-inducible factor (HIF) under normoxic conditions. Involved in transcriptional repression through interaction with HIF1A, HIF1AN and histone deacetylases. Ubiquitinates, in an oxygen-responsive manner, ADRB2. Acts as a negative regulator of mTORC1 by promoting ubiquitination and degradation of RPTOR. |
| Protein Name | Von Hippel-Lindau Disease Tumor Suppressor Protein G7 Pvhl |
| Database Links | Reactome: R-HSA-1234176 Reactome: R-HSA-3232142 Reactome: R-HSA-8951664 Reactome: R-HSA-9682706 Reactome: R-HSA-9694686 Reactome: R-HSA-9706019 Reactome: R-HSA-983168 |
| Cellular Localisation | Isoform 1: Cytoplasm Cell Membrane Peripheral Membrane Protein Endoplasmic Reticulum Nucleus Found Predominantly In The Cytoplasm And With Less Amounts Nuclear Or Membrane-Associated Colocalizes With Adrb2 At The Cell Membrane Isoform 3: Cytoplasm Equally Distributed Between The Nucleus And The Cytoplasm But Not Membrane-Associated |
| Alternative Antibody Names | Anti-Von Hippel-Lindau Disease Tumor Suppressor antibody Anti-Protein G7 antibody Anti-Pvhl antibody Anti-VHL antibody |
Information sourced from Uniprot.org