• Immunofluorescence analysis of U-251 MG cells using VHL Rabbit polyclonal antibody (STJ26089) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of VHL in paraffin-embedded human kidney using VHL Rabbit polyclonal antibody (STJ26089) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using VHL Rabbit polyclonal antibody (STJ26089) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-VHL antibody (167-186) (STJ26089)

SKU:
STJ26089

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-VHL (167-186) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: VHL
Gene ID: 7428
Uniprot ID: VHL_HUMAN
Immunogen Region: 167-186
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 167-186 of VHL (NP_000542.1).
Immunogen Sequence: RSLVKPENYRRLDIVRSLYE
Tissue Specificity Expressed in the adult and fetal brain and kidney.
Function Involved in the ubiquitination and subsequent proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Seems to act as a target recruitment subunit in the E3 ubiquitin ligase complex and recruits hydroxylated hypoxia-inducible factor (HIF) under normoxic conditions. Involved in transcriptional repression through interaction with HIF1A, HIF1AN and histone deacetylases. Ubiquitinates, in an oxygen-responsive manner, ADRB2. Acts as a negative regulator of mTORC1 by promoting ubiquitination and degradation of RPTOR.
Protein Name Von Hippel-Lindau Disease Tumor Suppressor
Protein G7
Pvhl
Database Links Reactome: R-HSA-1234176
Reactome: R-HSA-3232142
Reactome: R-HSA-8951664
Reactome: R-HSA-9682706
Reactome: R-HSA-9694686
Reactome: R-HSA-9706019
Reactome: R-HSA-983168
Cellular Localisation Isoform 1: Cytoplasm
Cell Membrane
Peripheral Membrane Protein
Endoplasmic Reticulum
Nucleus
Found Predominantly In The Cytoplasm And With Less Amounts Nuclear Or Membrane-Associated
Colocalizes With Adrb2 At The Cell Membrane
Isoform 3: Cytoplasm
Equally Distributed Between The Nucleus And The Cytoplasm But Not Membrane-Associated
Alternative Antibody Names Anti-Von Hippel-Lindau Disease Tumor Suppressor antibody
Anti-Protein G7 antibody
Anti-Pvhl antibody
Anti-VHL antibody

Information sourced from Uniprot.org

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