Western blot analysis of various lysates using VEPH1 Rabbit pAb (STJ110808) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-VEPH1 antibody (448-547) (STJ110808)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ110808
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General Information

Short DescriptionRabbit polyclonal VEPH1 (448-547) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityHuman/Mouse
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolVEPH1
Gene ID79674
Uniprot IDMELT_HUMAN
Immunogen Region448-547
Immunogen SequenceKSLAFHTMLTKGVGSDDGED ENRGDIPASISLSEIDPLGQ GNDKLPFKTDTERSQLGESS VSYPNIIHIDSENLSETVKE NSQEETPETTASPIEYQDKL
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 448-547 of human VEPH1 (NP_078897.2).

Additional Info

Function Interacts with TGF-beta receptor type-1 (TGFBR1) and inhibits dissociation of activated SMAD2 from TGFBR1, impeding its nuclear accumulation and resulting in impaired TGF-beta signaling. May also affect FOXO, Hippo and Wnt signaling.
Protein Name Ventricular Zone-Expressed Ph Domain-Containing Protein Homolog 1
Protein Melted
Database Links
Cellular Localisation Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Alternative Antibody Names Anti-Ventricular Zone-Expressed Ph Domain-Containing Protein Homolog 1 antibody
Anti-Protein Melted antibody
Anti-VEPH1 antibody
Anti-KIAA1692 antibody
Anti-VEPH antibody

Information sourced from Uniprot.org

Citations

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