• Immunohistochemistry analysis of VEGFB in paraffin-embedded human esophageal cancer using VEGFB Rabbit polyclonal antibody (STJ114562) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of VEGFB in paraffin-embedded rat kidney using VEGFB Rabbit polyclonal antibody (STJ114562) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using VEGFB Rabbit polyclonal antibody (STJ114562) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-VEGFB antibody (1-100) (STJ114562)

SKU:
STJ114562

$151.88 - $705.38
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-VEGFB (1-100) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: VEGFB
Gene ID: 7423
Uniprot ID: VEGFB_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of mouse VEGFB (NP_035827.1).
Immunogen Sequence: MSPLLRRLLLVALLQLARTQ APVSQFDGPSHQKKVVPWID VYARATCQPREVVVPLSMEL MGNVVKQLVPSCVTVQRCGG CCPDDGLECVPTGQHQVRMQ
Tissue Specificity Expressed in all tissues except liver. Highest levels found in heart, skeletal muscle and pancreas.
Post Translational Modifications VEGF-B186 is O-glycosylated.
Function Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis.
Protein Name Vascular Endothelial Growth Factor B
Vegf-B
Vegf-Related Factor
Vrf
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-194313
Reactome: R-HSA-195399
Cellular Localisation Secreted
Secreted But Remains Associated To Cells Or To The Extracellular Matrix Unless Released By Heparin
Alternative Antibody Names Anti-Vascular Endothelial Growth Factor B antibody
Anti-Vegf-B antibody
Anti-Vegf-Related Factor antibody
Anti-Vrf antibody
Anti-VEGFB antibody
Anti-VRF antibody

Information sourced from Uniprot.org

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