Anti-VEGFA antibody (110-159 aa) (STJ96235)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human VEGF-A at the amino acid range 110-159
General Information
| Short Description | Rabbit polyclonal anti-Vascular endothelial growth factor A, long form (110-159 aa) for use in WB, IF, IHC and ELISA in Human, Mouse, Rat, Pig and Rabbit samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/IHC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat/Pig/Rabbit |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IF 1:50-200IHC 1:100-1:300ELISA 1:10000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | VEGFA |
| Gene ID | 7422 |
| Uniprot ID | VEGFA_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human VEGF-A at the amino acid range 110-159 |
| Immunogen Region | 110-159 aa |
| Specificity | VEGF-A Polyclonal Antibody detects endogenous levels of VEGF-A protein. |
Additional Info
| Post Translational Modifications | Vascular endothelial growth factor A, long form: Produced by use of an alternative upstream CUG codon and post-translationally processed into the N-terminal N-VEGF form and the C-terminal secreted VEGFA form. |
| Function | N-VEGF: Participates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A. Involved in protecting cells from hypoxia-mediated cell death. VEGFA: Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Binds to the NRP1/neuropilin-1 receptor. Binding to NRP1 initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development. Also binds the DEAR/FBXW7-AS1 receptor. Isoform VEGF165B: Binds to the KDR receptor but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. |
| Protein Name | Vascular Endothelial Growth Factor A - Long FormL-VegfVascular Permeability FactorVpf Cleaved Into - N-Vegf - Vegfa |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-1234158Reactome: R-HSA-194138Reactome: R-HSA-194313Reactome: R-HSA-195399Reactome: R-HSA-4420097 P15692-4Reactome: R-HSA-5218921 P15692-4Reactome: R-HSA-6785807Reactome: R-HSA-8866910Reactome: R-HSA-9679191 P15692-4 |
| Cellular Localisation | N-Vegf: CytoplasmNucleusCytoplasmic In Normoxic Conditions And Localizes To The Nucleus Under Hypoxic ConditionsVegfa: SecretedIsoform L-Vegf189: Endoplasmic ReticulumGolgi ApparatusSecretedExtracellular SpaceExtracellular MatrixIsoform Vegf121: SecretedIsoform Vegf165: SecretedIsoform Vegf189: SecretedCell-Associated After Secretion And Is Bound Avidly By Heparin And The Extracellular MatrixAlthough It May Be Released As A Soluble Form By HeparinHeparinase Or Plasmin |
| Alternative Antibody Names | Anti-Vascular Endothelial Growth Factor A - Long Form antibodyAnti-L-Vegf antibodyAnti-Vascular Permeability Factor antibodyAnti-Vpf Cleaved Into - N-Vegf - Vegfa antibodyAnti-VEGFA antibodyAnti-VEGF antibody |
Information sourced from Uniprot.org