• Immunohistochemistry analysis of VEGF-A antibody in paraffin-embedded human skeletal muscle tissue.
  • Immunofluorescence analysis of mouse-spleen tissue. 1, VEGF-A Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunohistochemical analysis of paraffin-embedded Human skeletal muscle. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Immunofluorescence analysis of mouse-kidney tissue. 1, VEGF-A Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-VEGFA antibody (110-159 aa) (STJ96235)

SKU:
STJ96235

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IF/IHC/ELISA
Reactivity: Human/Mouse/Rat/Pig/Rabbit
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Vascular endothelial growth factor A, long form (110-159 aa) is suitable for use in Western Blot, Immunofluorescence, Immunohistochemistry and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IF 1:50-200
IHC 1:100-1:300
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: VEGFA
Gene ID: 7422
Uniprot ID: VEGFA_HUMAN
Immunogen Region: 110-159 aa
Specificity: VEGF-A Polyclonal Antibody detects endogenous levels of VEGF-A protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human VEGF-A at the amino acid range 110-159
Post Translational Modifications Vascular endothelial growth factor A, long form: Produced by use of an alternative upstream CUG codon and post-translationally processed into the N-terminal N-VEGF form and the C-terminal secreted VEGFA form.
Function N-VEGF: Participates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A. Involved in protecting cells from hypoxia-mediated cell death. VEGFA: Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Binds to the NRP1/neuropilin-1 receptor. Binding to NRP1 initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development. Also binds the DEAR/FBXW7-AS1 receptor. Isoform VEGF165B: Binds to the KDR receptor but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
Protein Name Vascular Endothelial Growth Factor A - Long Form
L-Vegf
Vascular Permeability Factor
Vpf Cleaved Into - N-Vegf - Vegfa
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-1234158
Reactome: R-HSA-194138
Reactome: R-HSA-194313
Reactome: R-HSA-195399
Reactome: R-HSA-4420097 P15692-4
Reactome: R-HSA-5218921 P15692-4
Reactome: R-HSA-6785807
Reactome: R-HSA-8866910
Reactome: R-HSA-9679191 P15692-4
Cellular Localisation N-Vegf: Cytoplasm
Nucleus
Cytoplasmic In Normoxic Conditions And Localizes To The Nucleus Under Hypoxic Conditions
Vegfa: Secreted
Isoform L-Vegf189: Endoplasmic Reticulum
Golgi Apparatus
Secreted
Extracellular Space
Extracellular Matrix
Isoform Vegf121: Secreted
Isoform Vegf165: Secreted
Isoform Vegf189: Secreted
Cell-Associated After Secretion And Is Bound Avidly By Heparin And The Extracellular Matrix
Although It May Be Released As A Soluble Form By Heparin
Heparinase Or Plasmin
Alternative Antibody Names Anti-Vascular Endothelial Growth Factor A - Long Form antibody
Anti-L-Vegf antibody
Anti-Vascular Permeability Factor antibody
Anti-Vpf Cleaved Into - N-Vegf - Vegfa antibody
Anti-VEGFA antibody
Anti-VEGF antibody

Information sourced from Uniprot.org

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