• Immunohistochemistry analysis of paraffin-embedded human thyroid gland tissue, using Vitamin D Receptor Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of lysates from MCF-7 cells, using Vitamin D Receptor Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunohistochemical analysis of paraffin-embedded Human colon. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunofluorescence analysis of HeLa cells, using Vitamin D Receptor Antibody. The picture on the right is blocked with the synthesized peptide.
  • Immunohistochemical analysis of paraffin-embedded Human colon. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Human colon. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Western blot analysis of various cells using VDR Polyclonal Antibody diluted at 1：500. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-VDR antibody (181-230 aa) (STJ96233)

SKU:
STJ96233

£48.00 - £252.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Vitamin D3 receptor (181-230 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: VDR
Gene ID: 7421
Uniprot ID: VDR_HUMAN
Immunogen Region: 181-230 aa
Specificity: VDR Polyclonal Antibody detects endogenous levels of VDR protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Vitamin D Receptor at the amino acid range 181-230
Function Nuclear receptor for calcitriol, the active form of vitamin D3 which mediates the action of this vitamin on cells. Enters the nucleus upon vitamin D3 binding where it forms heterodimers with the retinoid X receptor/RXR. The VDR-RXR heterodimers bind to specific response elements on DNA and activate the transcription of vitamin D3-responsive target genes. Plays a central role in calcium homeostasis. Also functions as a receptor for the secondary bile acid lithocholic acid (LCA) and its metabolites.
Protein Name Vitamin D3 Receptor
Vdr
1 -25-Dihydroxyvitamin D3 Receptor
Nuclear Receptor Subfamily 1 Group I Member 1
Database Links Reactome: R-HSA-196791
Reactome: R-HSA-383280
Reactome: R-HSA-4090294
Cellular Localisation Nucleus
Cytoplasm
Localizes Mainly To The Nucleus
Translocated Into The Nucleus Via Both Ligand-Dependent And Ligand-Independent Pathways
Ligand-Independent Nuclear Translocation Is Mediated By Ipo4
Alternative Antibody Names Anti-Vitamin D3 Receptor antibody
Anti-Vdr antibody
Anti-1 -25-Dihydroxyvitamin D3 Receptor antibody
Anti-Nuclear Receptor Subfamily 1 Group I Member 1 antibody
Anti-VDR antibody
Anti-NR1I1 antibody

Information sourced from Uniprot.org

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