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Anti-VCL antibody (786-835 aa) (STJ96247)

Western blot analysis of lysates from HeLa cells, treated with forskolin 40nM 30', using Vinculin Antibody. The lane on the right is blocked with the synthesized peptide.
Immunofluorescence analysis of A549 cells, using Vinculin Antibody. The picture on the right is blocked with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Vinculin Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of various cells using Vinculin Polyclonal Antibody. Secondary antibody was diluted at 1:20000

Anti-VCL antibody (786-835 aa) (STJ96247)

SKU:

STJ96247

£46.00 - £226.00

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General Information
Product Properties
Target Information
Additional Info
Warranty Information

Host:	Rabbit
Applications:	WB/IHC/IF/ELISA
Reactivity:	Human/Mouse/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit polyclonal antibody anti-Vinculin (786-835 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification:	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:	1 mg/mL
Dilution Range:	WB 1:500-1:2000 IHC-P 1:100-300 ELISA 1:20000 IF 1:100-300
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	VCL
Gene ID:	7414
Uniprot ID:	VINC_HUMAN
Immunogen Region:	786-835 aa
Specificity:	Vinculin Polyclonal Antibody detects endogenous levels of Vinculin protein.
Immunogen:	The antiserum was produced against synthesized peptide derived from the human Vinculin at the amino acid range 786-835

Post Translational Modifications	Phosphorylated.on serines, threonines and tyrosines. Phosphorylation on Tyr-1133 in activated platelets affects head-tail interactions and cell spreading but has no effect on actin binding nor on localization to focal adhesion plaques. Acetylated.mainly by myristic acid but also by a small amount of palmitic acid.
Function	Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.
Protein Name	Vinculin Metavinculin Mv
Database Links	Reactome: R-HSA-114608 Reactome: R-HSA-445355 Reactome: R-HSA-5674135 Reactome: R-HSA-6798695 Reactome: R-HSA-6802946 Reactome: R-HSA-6802948 Reactome: R-HSA-6802952 Reactome: R-HSA-6802955 Reactome: R-HSA-9649948 Reactome: R-HSA-9656223 Reactome: R-HSA-9725370
Cellular Localisation	Cell Membrane Peripheral Membrane Protein Cytoplasmic Side Cell Junction Adherens Junction Focal Adhesion Cytoplasm Cytoskeleton Sarcolemma Cell Projection Podosome Recruitment To Cell-Cell Junctions Occurs In A Myosin Ii-Dependent Manner Interaction With Ctnnb1 Is Necessary For Its Localization To The Cell-Cell Junctions
Alternative Antibody Names	Anti-Vinculin antibody Anti-Metavinculin antibody Anti-Mv antibody Anti-VCL antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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