| Host: | Rabbit |
| Applications: | ELISA/WB |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal anti-Vav3 for use in ELISA and WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Purification: | Antigen Affinity Purified |
| Dilution Range: | WB 1:1000 |
| Storage Instruction: | Storage at-20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at-20°C. |
| Gene Symbol: | VAV3 |
| Gene ID: | 10451 |
| Uniprot ID: | VAV3_HUMAN |
| Specificity: | The antibody detects a 98 kDa* protein corresponding to the molecular mass of Vav3 on SDS-PAGE immunoblots of human Jurkat cells. The antibody also strongly detects an unidentified protein at 120 kDa in Jurkat and K562. |
| Immunogen: | A synthetic peptide (coupled to KLH) corresponding to amino acid residues in the Dbl homology domain of human Vav3. This sequence has high homology with similar regions in rat and mouse Vav3, and has low homology to other Vav family members. |
| Immunogen Sequence: | Human |
| Tissue Specificity | Isoform 1 and isoform 3 are widely expressed.both are expressed at very low levels in skeletal muscle. In keratinocytes, isoform 1 is less abundant than isoform 3. Isoform 3 is detected at very low levels, if any, in adrenal gland, bone marrow, spleen, fetal brain and spinal cord.in these tissues, isoform 1 is readily detectable. |
| Post Translational Modifications | Phosphorylated. Phosphorylation can be mediated by ROS1. In osteoclasts, undergoes tyrosine phosphorylation in response to CSF1. |
| Function | Exchange factor for GTP-binding proteins RhoA, RhoG and, to a lesser extent, Rac1. Binds physically to the nucleotide-free states of those GTPases. Plays an important role in angiogenesis. Its recruitment by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly. May be important for integrin-mediated signaling, at least in some cell types. In osteoclasts, along with SYK tyrosine kinase, required for signaling through integrin alpha-v/beta-1 (ITAGV-ITGB1), a crucial event for osteoclast proper cytoskeleton organization and function. This signaling pathway involves RAC1, but not RHO, activation. Necessary for proper wound healing. In the course of wound healing, required for the phagocytotic cup formation preceding macrophage phagocytosis of apoptotic neutrophils. Responsible for integrin beta-2 (ITGB2)-mediated macrophage adhesion and, to a lesser extent, contributes to beta-3 (ITGB3)-mediated adhesion. Does not affect integrin beta-1 (ITGB1)-mediated adhesion. |
| Protein Name | Guanine Nucleotide Exchange Factor Vav3Vav-3 |
| Database Links | Reactome: R-HSA-114604Reactome: R-HSA-193648Reactome: R-HSA-2029482Reactome: R-HSA-2424491Reactome: R-HSA-2871796Reactome: R-HSA-2871809Reactome: R-HSA-3928665Reactome: R-HSA-416482Reactome: R-HSA-4420097Reactome: R-HSA-5218920Reactome: R-HSA-8980692Reactome: R-HSA-9013148Reactome: R-HSA-9013149Reactome: R-HSA-9013404Reactome: R-HSA-9013408Reactome: R-HSA-9664422Reactome: R-HSA-9748787 |
| Cellular Localisation | |
| Alternative Antibody Names | Anti-Guanine Nucleotide Exchange Factor Vav3 antibodyAnti-Vav-3 antibodyAnti-VAV3 antibody |
Information sourced from Uniprot.org
