• Immunofluorescence analysis of L929 cells using VARS Polyclonal Antibody (STJ26071) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using VARS antibody (STJ26071) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human breast cancer using VARS antibody (STJ26071) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using VARS antibody (STJ26071) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using VARS antibody (STJ26071) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-VARS antibody (1-280) (STJ26071)

SKU:
STJ26071

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-VARS (1-280) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: VARS1
Gene ID: 7407
Uniprot ID: SYVC_HUMAN
Immunogen Region: 1-280
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human VARS (NP_006286.1).
Immunogen Sequence: MSTLYVSPHPDAFPSLRALI AARYGEAGEGPGWGGAHPRI CLQPPPTSRTPFPPPRLPAL EQGPGGLWVWGATAVAQLLW PAGLGGPGGSRAAVLVQQWV SYADTELIPAACGATLPALG LRSSAQDPQAVLGALGRALS PLEEWLRLHTYLAGEAPTLA DLAAVTALLLPFRYVLDPPA RRIWNNVTRWFVTCVRQPEF RAVLGEVVLYSGARPLSHQP GPEAPALPKTAAQLKKEAK
Function Catalyzes the attachment of valine to tRNA(Val).
Protein Name Valine--Trna Ligase
Protein G7a
Valyl-Trna Synthetase
Valrs
Database Links Reactome: R-HSA-379716
Alternative Antibody Names Anti-Valine--Trna Ligase antibody
Anti-Protein G7a antibody
Anti-Valyl-Trna Synthetase antibody
Anti-Valrs antibody
Anti-VARS1 antibody
Anti-G7A antibody
Anti-VARS antibody
Anti-VARS2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance