| Host: |
Rabbit |
| Applications: |
WB/IHC-P/ELISA |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:2000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
VAPA |
| Gene ID: |
9218 |
| Uniprot ID: |
VAPA_HUMAN |
| Immunogen Region: |
137-227 |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 137-227 of human VAPA (NP_919415.2). |
| Immunogen Sequence: |
DKLNDMEPSKAVPLNASKQD GPMPKPHSVSLNDTETRKLM EECKRLQGEMMKLSEENRHL RDEGLRLRKVAHSDKPGSTS TASFRDNVTSP |
| Tissue Specificity | Ubiquitous. |
| Function | Endoplasmic reticulum (ER)-anchored protein that mediates the formation of contact sites between the ER and endosomes via interaction with FFAT motif-containing proteins such as STARD3 or WDR44. STARD3-VAPA interaction enables cholesterol transfer from the ER to endosomes. Via interaction with WDR44 participates in neosynthesized protein export. In addition, recruited to the plasma membrane through OSBPL3 binding. The OSBPL3-VAPA complex stimulates RRAS signaling which in turn attenuates integrin beta-1 (ITGB1) activation at the cell surface. With OSBPL3, may regulate ER morphology. May play a role in vesicle trafficking. |
| Protein Name | Vesicle-Associated Membrane Protein-Associated Protein AVamp-AVamp-Associated Protein AVap-A33 Kda Vamp-Associated ProteinVap-33 |
| Database Links | Reactome: R-HSA-1660661Reactome: R-HSA-6798695Reactome: R-HSA-9609523 |
| Cellular Localisation | Endoplasmic Reticulum MembraneSingle-Pass Type Iv Membrane ProteinCell MembraneCell JunctionTight JunctionNucleus MembranePresent In The Plasma Membrane And In Intracellular VesiclesTogether With Snare ProteinsMay Also Associate With The CytoskeletonColocalizes With Ocln At The Tight Junction In Polarized Epithelial Cells |
| Alternative Antibody Names | Anti-Vesicle-Associated Membrane Protein-Associated Protein A antibodyAnti-Vamp-A antibodyAnti-Vamp-Associated Protein A antibodyAnti-Vap-A antibodyAnti-33 Kda Vamp-Associated Protein antibodyAnti-Vap-33 antibodyAnti-VAPA antibodyAnti-VAP33 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and CHCHD3 knockout (KO) 293T cells, using [KO Validated] CHCHD3 Rabbit polyclonal antibody (STJ11100835) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89770/358242/STJ11100835_1__03090.1713122575.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and CHCHD3 knockout (KO) 293T cells, using [KO Validated] CHCHD3 Rabbit polyclonal antibody (STJ11100835) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
