• Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-VAMP8 antibody (46-96 aa) (STJ196538)

SKU:
STJ196538

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Vesicle-associated membrane protein 8 (46-96 aa) is suitable for use in Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IHC-P 1:50-200
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: VAMP8
Gene ID: 8673
Uniprot ID: VAMP8_HUMAN
Immunogen Region: 46-96 aa
Specificity: This antibody detects endogenous levels of VAMP8 at Human/Mouse/Rat
Immunogen: Synthesized peptide derived from the human VAMP8 at the amino acid range 46-96
Post Translational Modifications (Microbial infection) Stearoylated By S.flexneri N-epsilon-fatty acyltransferase IcsB, thereby disrupting the host actin cytoskeleton.
Function SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. VAMP8 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane via its interaction with the STX17-SNAP29 binary t-SNARE complex. Also required for dense-granule secretion in platelets. Also plays a role in regulated enzyme secretion in pancreatic acinar cells. Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells. Involved in the homotypic fusion of early and late endosomes. Participates also in the activation of type I interferon antiviral response through a TRIM6-dependent mechanism.
Protein Name Vesicle-Associated Membrane Protein 8
Vamp-8
Endobrevin
Edb
Database Links Reactome: R-HSA-1236974
Reactome: R-HSA-199992
Reactome: R-HSA-432720
Reactome: R-HSA-432722
Reactome: R-HSA-6798695
Reactome: R-HSA-8856825
Reactome: R-HSA-8856828
Cellular Localisation Lysosome Membrane
Single-Pass Type Iv Membrane Protein
Early Endosome Membrane
Late Endosome Membrane
Cell Membrane
Zymogen Granule Membrane
Perinuclear Vesicular Structures Of The Early And Late Endosomes
Coated Pits
And Trans-Golgi
Sub-Tight Junctional Domain In Retinal Pigment Epithelium Cells
Midbody Region During Cytokinesis
Lumenal Oriented
Apical Membranes Of Nephric Tubular Cell
Cycles Through The Apical But Not Through The Basolateral Plasma Membrane
Apical Region Of Acinar Cells
In Zymogen Granule Membranes
Alternative Antibody Names Anti-Vesicle-Associated Membrane Protein 8 antibody
Anti-Vamp-8 antibody
Anti-Endobrevin antibody
Anti-Edb antibody
Anti-VAMP8 antibody

Information sourced from Uniprot.org

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