| Host: | Rabbit |
| Applications: | IHC/IF |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Vesicle-associated membrane protein 8 (46-96 aa) is suitable for use in Immunohistochemistry and Immunofluorescence research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | IHC-P 1:50-200 IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | VAMP8 |
| Gene ID: | 8673 |
| Uniprot ID: | VAMP8_HUMAN |
| Immunogen Region: | 46-96 aa |
| Specificity: | This antibody detects endogenous levels of VAMP8 at Human/Mouse/Rat |
| Immunogen: | Synthesized peptide derived from the human VAMP8 at the amino acid range 46-96 |
| Post Translational Modifications | (Microbial infection) Stearoylated By S.flexneri N-epsilon-fatty acyltransferase IcsB, thereby disrupting the host actin cytoskeleton. |
| Function | SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. VAMP8 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane via its interaction with the STX17-SNAP29 binary t-SNARE complex. Also required for dense-granule secretion in platelets. Also plays a role in regulated enzyme secretion in pancreatic acinar cells. Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells. Involved in the homotypic fusion of early and late endosomes. Participates also in the activation of type I interferon antiviral response through a TRIM6-dependent mechanism. |
| Protein Name | Vesicle-Associated Membrane Protein 8 Vamp-8 Endobrevin Edb |
| Database Links | Reactome: R-HSA-1236974 Reactome: R-HSA-199992 Reactome: R-HSA-432720 Reactome: R-HSA-432722 Reactome: R-HSA-6798695 Reactome: R-HSA-8856825 Reactome: R-HSA-8856828 |
| Cellular Localisation | Lysosome Membrane Single-Pass Type Iv Membrane Protein Early Endosome Membrane Late Endosome Membrane Cell Membrane Zymogen Granule Membrane Perinuclear Vesicular Structures Of The Early And Late Endosomes Coated Pits And Trans-Golgi Sub-Tight Junctional Domain In Retinal Pigment Epithelium Cells Midbody Region During Cytokinesis Lumenal Oriented Apical Membranes Of Nephric Tubular Cell Cycles Through The Apical But Not Through The Basolateral Plasma Membrane Apical Region Of Acinar Cells In Zymogen Granule Membranes |
| Alternative Antibody Names | Anti-Vesicle-Associated Membrane Protein 8 antibody Anti-Vamp-8 antibody Anti-Endobrevin antibody Anti-Edb antibody Anti-VAMP8 antibody |
Information sourced from Uniprot.org