| Host: | Mouse |
| Applications: | ELISA/WB/ICC/IF |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal anti-VAMP-2 (1-89aa) for use in ELISA, WB, ICC and IF in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Clonality : | Monoclonal |
| Clone ID : | 3E5 |
| Conjugation: | Unconjugated |
| Isotype: | IgG1k |
| Formulation: | Liquid in phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol |
| Purification: | By protein-A affinity chromatography |
| Concentration: | 1 mg/mL |
| Storage Instruction: | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | VAMP2 |
| Gene ID: | 6844 |
| Uniprot ID: | VAMP2_HUMAN |
| Immunogen Region: | 1-89aa |
| Accession Number: | NP_055047 |
| Immunogen: | Recombinant human Synaptobrevin 2 (1-89aa) purified from E. coli |
| Tissue Specificity | Nervous system and skeletal muscle. |
| Post Translational Modifications | Phosphorylated by PRKCZ in vitro and this phosphorylation is increased in the presence of WDFY2. (Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type B (BoNT/B, botB) which hydrolyzes the 76-Gln-|-Phe-77 bond and probably inhibits neurotransmitter release. (Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type D (BoNT/D, botD) which probably hydrolyzes the 59-Lys-|-Leu-60 bond and inhibits neurotransmitter release. Note that humans are not known to be infected by C.botulinum type D. (Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type F (BoNT/F, botF) which hydrolyzes the 58-Gln-|-Lys-59 bond and probably inhibits neurotransmitter release. (Microbial infection) Targeted and hydrolyzed by C.tetani tetanus toxin (tetX) which hydrolyzes the 76-Gln-|-Phe-77 bond and probably inhibits neurotransmitter release. |
| Function | Involved in the targeting and/or fusion of transport vesicles to their target membrane. Major SNARE protein of synaptic vesicles which mediates fusion of synaptic vesicles to release neurotransmitters. Essential for fast vesicular exocytosis and activity-dependent neurotransmitter release as well as fast endocytosis that mediates rapid reuse of synaptic vesicles. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1. |
| Protein Name | Vesicle-Associated Membrane Protein 2Vamp-2Synaptobrevin-2 |
| Database Links | Reactome: R-HSA-1445148Reactome: R-HSA-181429Reactome: R-HSA-181430Reactome: R-HSA-199992Reactome: R-HSA-210500Reactome: R-HSA-212676Reactome: R-HSA-264642Reactome: R-HSA-264876Reactome: R-HSA-422356Reactome: R-HSA-432720Reactome: R-HSA-432722Reactome: R-HSA-449836Reactome: R-HSA-5250955Reactome: R-HSA-5250958Reactome: R-HSA-5250981Reactome: R-HSA-5250982Reactome: R-HSA-5250989Reactome: R-HSA-8856825Reactome: R-HSA-8856828Reactome: R-HSA-888590Reactome: R-HSA-9609523Reactome: R-HSA-9662360 |
| Cellular Localisation | Cytoplasmic VesicleSecretory VesicleSynaptic Vesicle MembraneSingle-Pass Type Iv Membrane ProteinCell MembraneColocalizes With Prkcz And Wdfy2 In Intracellular Vesicles |
| Alternative Antibody Names | Anti-Vesicle-Associated Membrane Protein 2 antibodyAnti-Vamp-2 antibodyAnti-Synaptobrevin-2 antibodyAnti-VAMP2 antibodyAnti-SYB2 antibody |
Information sourced from Uniprot.org