• Western blot analysis of extracts of normal 293T cells and 293T transfected with V5 Protein, using rabbit anti V5-Tag antibody (STJ11103713) at 1:20000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Chromatin immunoprecipitation was performed with 20 Mu g of cross-linked chromatin from 293F cells (left) and 293F cells transfected with a GATA3 expression vector containing a single C-terminal V5-Tag (right) , using 5 Mu g of Rabbit anti V5-Tag monoclonal antibody (STJ11103713) and Rabbit IgG isotype control. The enrichment of immunoprecipitated DNA at different genomic loci was examined by quantitative PCR. The histogram compares the ratio of the immunoprecipitated DNA to the input at given loci.
  • Western blot analysis of extracts of 293T cells, using rabbit anti V5-Tag antibody (STJ11103713) at 1:10000-1:320000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunoprecipitation analysis of 300 Mu g extracts of CHO-V5 cells using 30ug Rabbit anti V5-Tag antibody (STJ11103713). Western blot was performed from the immunoprecipitate using Rabbit anti V5-Tag antibody (STJ11103713) at a dilution of 1:3000.
  • Immunofluorescence analysis of 293T cells transfected with V5 (mCherry) protein and untreated 293T cells using rabbit anti V5-Tag monoclonal antibody (STJ11103713) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of 293T cells transfected with V5 (mCherry) protein and untreated 293T cells using Rabbit anti V5-Tag monoclonal antibody (STJ11103713) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunoprecipitation analysis of 300ug extracts of CHO-V5 cells using 3ug rabbit anti V5-Tag antibody (STJ11103713). Western blot was performed from the immunoprecipitate using rabbit anti V5-Tag antibody (STJ11103713) at a dilition of 1:5000.
  • Western blot analysis of lysates from CHO cells, using Rabbit anti V5-Tag monoclonal antibody (STJ11103713) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-V5-Tag antibody [S3MR] (STJ11103713)

SKU:
STJ11103713

£135.50 - £249.50
Current Stock:
Host: Rabbit
Applications: WB/IF/ICC/IP/ELISA/ChIP
Reactivity: Species independent
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S3MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:2000-1:6000
IF/ICC:1:50-1:200
IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirement
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Specificity: A synthetic peptide corresponding to V5-Tag.
Immunogen Sequence: GKPIPNPLLGLDST
Background Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.

Information sourced from Uniprot.org

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