• Western blot analysis of extracts of normal 293T cells and 293T transfected with V5 Protein, using rabbit anti V5-Tag antibody (STJ11103713) at 1:20000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Chromatin immunoprecipitation was performed with 20 Mu g of cross-linked chromatin from 293F cells (left) and 293F cells transfected with a GATA3 expression vector containing a single C-terminal V5-Tag (right) , using 5 Mu g of Rabbit anti V5-Tag monoclonal antibody (STJ11103713) and Rabbit IgG isotype control. The enrichment of immunoprecipitated DNA at different genomic loci was examined by quantitative PCR. The histogram compares the ratio of the immunoprecipitated DNA to the input at given loci.
  • Western blot analysis of extracts of 293T cells, using rabbit anti V5-Tag antibody (STJ11103713) at 1:10000-1:320000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunoprecipitation analysis of 300 Mu g extracts of CHO-V5 cells using 30ug Rabbit anti V5-Tag antibody (STJ11103713). Western blot was performed from the immunoprecipitate using Rabbit anti V5-Tag antibody (STJ11103713) at a dilution of 1:3000.
  • Immunofluorescence analysis of 293T cells transfected with V5 (mCherry) protein and untreated 293T cells using rabbit anti V5-Tag monoclonal antibody (STJ11103713) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of 293T cells transfected with V5 (mCherry) protein and untreated 293T cells using Rabbit anti V5-Tag monoclonal antibody (STJ11103713) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunoprecipitation analysis of 300ug extracts of CHO-V5 cells using 3ug rabbit anti V5-Tag antibody (STJ11103713). Western blot was performed from the immunoprecipitate using rabbit anti V5-Tag antibody (STJ11103713) at a dilition of 1:5000.
  • Western blot analysis of lysates from CHO cells, using Rabbit anti V5-Tag monoclonal antibody (STJ11103713) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-V5-Tag antibody [S3MR] (STJ11103713)

SKU:
STJ11103713

€111.88 - €310.63
Current Stock:
Host: Rabbit
Applications: WB/IF/IP
Reactivity: Species independent
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-V5-Tag is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S3MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:2000-1:6000
IF/ICC 1:50-1:200
IP 1:1000-1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen: A synthetic peptide corresponding to V5-Tag.
Immunogen Sequence: GKPIPNPLLGLDST
Background This gene encodes a member of the kappa class of the glutathione transferase superfamily of enzymes that function in cellular detoxification. The encoded protein is localized to the peroxisome and catalyzes the conjugation of glutathione to a wide range of hydrophobic substates facilitating the removal of these compounds from cells. Alternative splicing results in multiple transcript variants.

Information sourced from Uniprot.org

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