• Immunofluorescence analysis of PC-12 cells using USP9X/USP9Y Rabbit polyclonal antibody (STJ11103439) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using USP9X/USP9Y Rabbit polyclonal antibody (STJ11103439) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HepG2 cells using USP9X/USP9Y Rabbit polyclonal antibody (STJ11103439) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of 293T cells using USP9X/USP9Y Rabbit polyclonal antibody (STJ11103439) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of USP9X/USP9Y in paraffin-embedded mouse kidney tissue using USP9X/USP9Y Rabbit polyclonal antibody (STJ11103439) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of USP9X/USP9Y in paraffin-embedded mouse testis tissue using USP9X/USP9Y Rabbit polyclonal antibody (STJ11103439) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of USP9X/USP9Y in paraffin-embedded rat kidney tissue using USP9X/USP9Y Rabbit polyclonal antibody (STJ11103439) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using USP9X/USP9Y Rabbit polyclonal antibody (STJ11103439) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-USP9Y antibody (2456-2555) (STJ11103439)

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STJ11103439

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-USP9Y (2456-2555) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: USP9Y
Gene ID: 8287
Uniprot ID: USP9Y_HUMAN
Immunogen Region: 2456-2555
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 2456-2555 of human USP9X/USP9Y (NP_004645.2).
Immunogen Sequence: YFLERSHSARMTLAKACELC PEEEPDDQDAPDEHEPSPSE DAPLYPHSPASQYQQNNHVH GQPYTGPAAHHLNNPQKTGQ RTQENYEGNEEVSSPQMKDQ
Tissue Specificity Widely expressed in embryonic and adult tissues.
Function May function as a ubiquitin-protein or polyubiquitin hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. May therefore play an important regulatory role at the level of protein turnover by preventing degradation of proteins through the removal of conjugated ubiquitin. Essential component of TGF-beta/BMP signaling cascade. Deubiquitinates monoubiquitinated SMAD4, opposing the activity of E3 ubiquitin-protein ligase TRIM33. Monoubiquitination of SMAD4 hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade. Deubiquitination of SMAD4 by USP9X re-empowers its competence to mediate TGF-beta signaling.
Protein Name Probable Ubiquitin Carboxyl-Terminal Hydrolase Faf-Y
Deubiquitinating Enzyme Faf-Y
Fat Facets Protein-Related - Y-Linked
Ubiquitin Thioesterase Faf-Y
Ubiquitin-Specific Protease 9 - Y Chromosome
Ubiquitin-Specific-Processing Protease Faf-Y
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Probable Ubiquitin Carboxyl-Terminal Hydrolase Faf-Y antibody
Anti-Deubiquitinating Enzyme Faf-Y antibody
Anti-Fat Facets Protein-Related - Y-Linked antibody
Anti-Ubiquitin Thioesterase Faf-Y antibody
Anti-Ubiquitin-Specific Protease 9 - Y Chromosome antibody
Anti-Ubiquitin-Specific-Processing Protease Faf-Y antibody
Anti-USP9Y antibody
Anti-DFFRY antibody

Information sourced from Uniprot.org

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