| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Rat/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Inactive ubiquitin carboxyl-terminal hydrolase 53 (951-1000 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-2000IHC 1:100-1:300ELISA 1:10000IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | USP53 |
| Gene ID: | 54532 |
| Uniprot ID: | UBP53_HUMAN |
| Immunogen Region: | 951-1000 aa |
| Specificity: | USP53 Polyclonal Antibody detects endogenous levels of USP53 protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human USP53 at the amino acid range 951-1000 |
| Function | Deubiquitinase that mediates 'Lys-63'-linked deubiquitination of tight junction proteins, such as MARVELD2 and LSR, and which is involved in the survival of auditory hair cells and hearing. Specifically cleaves 'Lys-63'-linked polyubiquitin chains composed of at least 3 ubiquitin molecules, while it is not able to deubiquitinate substrates with shorter ubiquitin chains: recognizes ubiquitin chain in position S2 and catalyzes en bloc cleavage of polyubiquitin chains from substrate proteins. Probably acts by modulating the barrier properties and mechanical stability of tight junctions via deubiquitination of MARVELD2 and LSR. |
| Protein Name | Ubiquitin Carboxyl-Terminal Hydrolase 53Ubiquitin-Specific Peptidase 53 |
| Database Links | |
| Cellular Localisation | Cell JunctionTight Junction |
| Alternative Antibody Names | Anti-Ubiquitin Carboxyl-Terminal Hydrolase 53 antibodyAnti-Ubiquitin-Specific Peptidase 53 antibodyAnti-USP53 antibodyAnti-KIAA1350 antibody |
Information sourced from Uniprot.org
