Anti-USP53 antibody (495-582) (STJ11105514)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal Inactive Ubiquitin Carboxyl-Terminal Hydrolase 53 (495-582) antibody for use in WB, IF, ICC and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | USP53 |
| Gene ID | 54532 |
| Uniprot ID | UBP53_HUMAN |
| Immunogen Region | 495-582 |
| Immunogen Sequence | APNGFKQHGNPHLYHSQGKG SYKHDRVVPQSRASAQIISS SKSQILAPGEKITGKVKSDN GTGYDTDSSQDSRDRGNSCD SSSKSRNR |
| Specificity | Recombinant Protein corresponding to a sequence within amino acids 495-582 of human USP53 (NP_061923.2). |
Additional Info
| Function | Deubiquitinase that mediates 'Lys-63'-linked deubiquitination of tight junction proteins, such as MARVELD2 and LSR, and which is involved in the survival of auditory hair cells and hearing. Specifically cleaves 'Lys-63'-linked polyubiquitin chains composed of at least 3 ubiquitin molecules, while it is not able to deubiquitinate substrates with shorter ubiquitin chains: recognizes ubiquitin chain in position S2 and catalyzes en bloc cleavage of polyubiquitin chains from substrate proteins. Probably acts by modulating the barrier properties and mechanical stability of tight junctions via deubiquitination of MARVELD2 and LSR. |
| Protein Name | Ubiquitin Carboxyl-Terminal Hydrolase 53Ubiquitin-Specific Peptidase 53 |
| Database Links | |
| Cellular Localisation | Cell JunctionTight Junction |
| Alternative Antibody Names | Anti-Ubiquitin Carboxyl-Terminal Hydrolase 53 antibodyAnti-Ubiquitin-Specific Peptidase 53 antibodyAnti-USP53 antibodyAnti-KIAA1350 antibody |
Information sourced from Uniprot.org