| Host: |
Goat |
| Applications: |
Pep-ELISA/WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-USP4/UNP (N-Term) is suitable for use in ELISA and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: |
0.5 mg/mL |
| Dilution Range: |
WB-1-3µg/mlELISA-antibody detection limit dilution 1:8000. |
| Storage Instruction: |
Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: |
USP4 |
| Gene ID: |
7375 |
| Uniprot ID: |
UBP4_HUMAN |
| Immunogen Region: |
N-Term |
| Accession Number: |
NP_003354.2; NP_955475.1 |
| Immunogen Sequence: |
AEGGGCRERPDAE |
| Post Translational Modifications | Phosphorylated at Ser-445 by PKB/AKT1 in response to EGF stimulus, promoting its ability deubiquitinate RHEB. Monoubiquitinated by TRIM21. Ubiquitination does not lead to its proteasomal degradation. Autodeubiquitinated. |
| Function | Deubiquitinating enzyme that removes conjugated ubiquitin from target proteins. Deubiquitinates PDPK1. Deubiquitinates TRIM21. Deubiquitinates receptor ADORA2A which increases the amount of functional receptor at the cell surface. Deubiquitinates HAS2. Deubiquitinates RHEB in response to EGF signaling, promoting mTORC1 signaling. May regulate mRNA splicing through deubiquitination of the U4 spliceosomal protein PRPF3. This may prevent its recognition by the U5 component PRPF8 thereby destabilizing interactions within the U4/U6.U5 snRNP. May also play a role in the regulation of quality control in the ER. |
| Protein Name | Ubiquitin Carboxyl-Terminal Hydrolase 4Deubiquitinating Enzyme 4Ubiquitin Thioesterase 4Ubiquitin-Specific-Processing Protease 4Ubiquitous Nuclear Protein Homolog |
| Database Links | Reactome: R-HSA-5357786Reactome: R-HSA-5357905Reactome: R-HSA-5357956Reactome: R-HSA-5689880 |
| Cellular Localisation | CytoplasmNucleusShuttles Between The Nucleus And CytoplasmExported To The Cytoplasm In A Crm1-Dependent Manner And Recycled Back To The Nucleus Via The Importin Alpha/Beta Heterodimeric Import ReceptorThe Relative Amounts Found In The Nucleus And Cytoplasm Vary According To The Cell Type |
| Alternative Antibody Names | Anti-Ubiquitin Carboxyl-Terminal Hydrolase 4 antibodyAnti-Deubiquitinating Enzyme 4 antibodyAnti-Ubiquitin Thioesterase 4 antibodyAnti-Ubiquitin-Specific-Processing Protease 4 antibodyAnti-Ubiquitous Nuclear Protein Homolog antibodyAnti-USP4 antibodyAnti-UNP antibodyAnti-UNPH antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and USP4 knockout (KO) HeLa cells, using [KO Validated] USP4 Rabbit polyclonal antibody (STJ11100881) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89815/358278/STJ11100881_1__46401.1713122625.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and USP4 knockout (KO) HeLa cells, using [KO Validated] USP4 Rabbit polyclonal antibody (STJ11100881) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
