Anti-USP30 antibody (31-80 aa) (STJ96199)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human USP30 at the amino acid range 31-80
General Information
| Short Description | Rabbit polyclonal anti-Ubiquitin carboxyl-terminal hydrolase 30 (31-80 aa) for use in WB and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000ELISA 1:10000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | USP30 |
| Gene ID | 84749 |
| Uniprot ID | UBP30_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human USP30 at the amino acid range 31-80 |
| Immunogen Region | 31-80 aa |
| Specificity | USP30 Polyclonal Antibody detects endogenous levels of USP30 protein. |
Additional Info
| Post Translational Modifications | Ubiquitinated by parkin (PRKN) at Lys-235 and Lys-289, leading to its degradation. |
| Function | Deubiquitinating enzyme tethered to the mitochondrial outer membrane that acts as a key inhibitor of mitophagy by counteracting the action of parkin (PRKN): hydrolyzes ubiquitin attached by parkin on target proteins, such as RHOT1/MIRO1 and TOMM20, thereby blocking parkin's ability to drive mitophagy. Preferentially cleaves 'Lys-6'- and 'Lys-11'-linked polyubiquitin chains, 2 types of linkage that participate in mitophagic signaling. Does not cleave efficiently polyubiquitin phosphorylated at 'Ser-65'. Acts as a negative regulator of mitochondrial fusion by mediating deubiquitination of MFN1 and MFN2. |
| Protein Name | Ubiquitin Carboxyl-Terminal Hydrolase 30Deubiquitinating Enzyme 30Ubiquitin Thioesterase 30Ubiquitin-Specific-Processing Protease 30Ub-Specific Protease 30 |
| Database Links | Reactome: R-HSA-5689880Reactome: R-HSA-9664873 |
| Cellular Localisation | Mitochondrion Outer Membrane |
| Alternative Antibody Names | Anti-Ubiquitin Carboxyl-Terminal Hydrolase 30 antibodyAnti-Deubiquitinating Enzyme 30 antibodyAnti-Ubiquitin Thioesterase 30 antibodyAnti-Ubiquitin-Specific-Processing Protease 30 antibodyAnti-Ub-Specific Protease 30 antibodyAnti-USP30 antibody |
Information sourced from Uniprot.org