Anti-USP16 antibody (1-280) (STJ28424)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal USP16 (1-280) antibody for use in WB and ELISA in human, mouse, rat and monkey samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat/Monkey |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | USP16 |
| Gene ID | 10600 |
| Uniprot ID | UBP16_HUMAN |
| Immunogen Region | 1-280 |
| Immunogen Sequence | MGKKRTKGKTVPIDDSSETL EPVCRHIRKGLEQGNLKKAL VNVEWNICQDCKTDNKVKDK AEEETEEKPSVWLCLKCGHQ GCGRNSQEQHALKHYLTPRS EPHCLVLSLDNWSVWCYVCD NEVQYCSSNQLGQVVDYVRK QASITTPKPEKDNGNIELEN KKLEKESKNEQEREKKENMA KENPPMNSPCQITVKGLSNL GNTCFFNAVMQNLSQTPVLR ELLKEVKMSGTIVKIEPPD |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human USP16 (NP_001001992.1). |
Additional Info
| Tissue Specificity | Present in all the tissues examined including fetal brain, lung, liver, kidney, and adult heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. |
| Post Translational Modifications | Phosphorylated at the onset of mitosis and dephosphorylated during the metaphase/anaphase transition. Phosphorylation by AURKB enhances the deubiquitinase activity. |
| Function | Specifically deubiquitinates 'Lys-120' of histone H2A (H2AK119Ub), a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A is a prerequisite for subsequent phosphorylation at 'Ser-11' of histone H3 (H3S10ph), and is required for chromosome segregation when cells enter into mitosis. In resting B- and T-lymphocytes, phosphorylation by AURKB leads to enhance its activity, thereby maintaining transcription in resting lymphocytes. Regulates Hox gene expression via histone H2A deubiquitination. Prefers nucleosomal substrates. Does not deubiquitinate histone H2B. Also deubiquitinates non-histone proteins, such as ribosomal protein RPS27A: deubiquitination of monoubiquitinated RPS27A promotes maturation of the 40S ribosomal subunit. Also mediates deubiquitination of tektin proteins (TEKT1, TEKT2, TEK3, TEKT4 and TEKT5), promoting their stability. |
| Protein Name | Ubiquitin Carboxyl-Terminal Hydrolase 16Deubiquitinating Enzyme 16Ubiquitin Thioesterase 16Ubiquitin-Processing Protease Ubp-MUbiquitin-Specific-Processing Protease 16 |
| Database Links | Reactome: R-HSA-5689880 |
| Cellular Localisation | NucleusCytoplasm |
| Alternative Antibody Names | Anti-Ubiquitin Carboxyl-Terminal Hydrolase 16 antibodyAnti-Deubiquitinating Enzyme 16 antibodyAnti-Ubiquitin Thioesterase 16 antibodyAnti-Ubiquitin-Processing Protease Ubp-M antibodyAnti-Ubiquitin-Specific-Processing Protease 16 antibodyAnti-USP16 antibodyAnti-MSTP039 antibody |
Information sourced from Uniprot.org