Anti-USP15 antibody (STJ11107771)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthetic peptide. This information is considered to be commercially sensitive.
General Information
| Short Description | Rabbit polyclonal Ubiquitin Carboxyl-Terminal Hydrolase 15 antibody for use in WB in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB, 1:1000-1:3000 |
| Formulation | PBS with 0.09% Sodium Azide, 50% Glycerol, pH7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | USP15 |
| Gene ID | 9958 |
| Uniprot ID | UBP15_HUMAN |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Immunogen Sequence | Human |
Additional Info
| Post Translational Modifications | Phosphorylated. Phosphorylation protects against ubiquitination and subsequent degradation by the proteasome. Ubiquitinated, leading to degradation by the proteasome. |
| Function | Hydrolase that removes conjugated ubiquitin from target proteins and regulates various pathways such as the TGF-beta receptor signaling, NF-kappa-B and RNF41/NRDP1-PRKN pathways. Acts as a key regulator of TGF-beta receptor signaling pathway, but the precise mechanism is still unclear: according to a report, acts by promoting deubiquitination of monoubiquitinated R-SMADs (SMAD1, SMAD2 and/or SMAD3), thereby alleviating inhibition of R-SMADs and promoting activation of TGF-beta target genes. According to another reports, regulates the TGF-beta receptor signaling pathway by mediating deubiquitination and stabilization of TGFBR1, leading to an enhanced TGF-beta signal. Able to mediate deubiquitination of monoubiquitinated substrates, 'Lys-27'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. May also regulate gene expression and/or DNA repair through the deubiquitination of histone H2B. Acts as an inhibitor of mitophagy by counteracting the action of parkin (PRKN): hydrolyzes cleavage of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains attached by parkin on target proteins such as MFN2, thereby reducing parkin's ability to drive mitophagy. Acts as an associated component of COP9 signalosome complex (CSN) and regulates different pathways via this association: regulates NF-kappa-B by mediating deubiquitination of NFKBIA and deubiquitinates substrates bound to VCP. Involved in endosome organization by mediating deubiquitination of SQSTM1: ubiquitinated SQSTM1 forms a molecular bridge that restrains cognate vesicles in the perinuclear region and its deubiquitination releases target vesicles for fast transport into the cell periphery. Acts as a negative regulator of antifungal immunity by mediating 'Lys-27'-linked deubiquitination of CARD9, thereby inactivating CARD9. (Microbial infection) Protects APC and human papillomavirus type 16 protein E6 against degradation via the ubiquitin proteasome pathway. |
| Protein Name | Ubiquitin Carboxyl-Terminal Hydrolase 15Deubiquitinating Enzyme 15Ubiquitin Thioesterase 15Ubiquitin-Specific-Processing Protease 15Unph-2Unph4 |
| Database Links | Reactome: R-HSA-2173788Reactome: R-HSA-5689603Reactome: R-HSA-5689880 |
| Cellular Localisation | CytoplasmNucleusMitochondrion |
| Alternative Antibody Names | Anti-Ubiquitin Carboxyl-Terminal Hydrolase 15 antibodyAnti-Deubiquitinating Enzyme 15 antibodyAnti-Ubiquitin Thioesterase 15 antibodyAnti-Ubiquitin-Specific-Processing Protease 15 antibodyAnti-Unph-2 antibodyAnti-Unph4 antibodyAnti-USP15 antibodyAnti-KIAA0529 antibody |
Information sourced from Uniprot.org