| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
USP12 |
| Gene ID: |
219333 |
| Uniprot ID: |
UBP12_HUMAN |
| Immunogen Region: |
1-100 |
| Specificity: |
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human USP12 (NP_872294.2). |
| Immunogen Sequence: |
MEILMTVSKFASICTMGANA SALEKEIGPEQFPVNEHYFG LVNFGNTCYCNSVLQALYFC RPFREKVLAYKSQPRKKESL LTCLADLFHSIATQKKKVGV |
| Function | Deubiquitinating enzyme that plays various roles in the regulation of the immune response and inflammation. During TCR engagement and activation, translocates into the cytoplasm and deubiquitinates its substrates LAT and TRAT1 and prevents their lysosome-dependent degradation to stabilize the TCR signaling complex at the plasma membrane. Plays an essential role in the selective LPS-induced macrophage response through the activation of NF-kappa-B pathway. In addition, promotes that antiviral immune response through targeting DNA sensor IFI16 to inhibit its proteasome-dependent degradation. Participates in the interferon signaling pathway and antiviral response independently of its deubiquitinase activity by maintaining nuclear phosphorylated STAT1 levels via inhibition of its CREBBP-mediated acetylation and subsequent dephosphorylation. Plays an intrinsic role in promoting the differentiation, activation and proliferation of CD4(+) T-cell by activating the NF-kappa-B signaling pathway through deubiquitinating and stabilizing B-cell lymphoma/leukemia 10/BCL10. In myeloid-derived suppressor cells promotes the activation of the NF-kappa-B via deubiquitination and stabilization of RELA. Regulates the 'Lys-63'-linked polyubiquitin chains of BAX and thereby modulates the mitochondrial apoptotic process. Negative regulator of NOTCH signaling that specifically deubiquitinates non-activated NOTCH receptors to target them for lysosomal degradation.deubiquitination of NOTCH stimulates its transport form late endosomes to lysosomes. Protects neurons against HTT/huntingtin-induced polyglutamine expansion-dependent neurodegeneration through regulation of autophagic flux. This function is independent of deubiquitinase activity or of other components of the USP12-WDR20-WDR48 deubiquitinating complex. In complex with WDR48, acts as a potential tumor suppressor by positively regulating PHLPP1 stability. (Microbial infection) Forms a complex with Epstein-Barr virus protein EBNA3 which is an active deubiquitinase activity that may select specific substrates to promote B-lymphocyte transformation. |
| Protein Name | Ubiquitin Carboxyl-Terminal Hydrolase 12Deubiquitinating Enzyme 12Ubiquitin Specific Peptidase 12Ubiquitin Thioesterase 12Ubiquitin-Hydrolyzing Enzyme 1Ubiquitin-Specific-Processing Protease 12 |
| Database Links | Reactome: R-HSA-5689880 |
| Cellular Localisation | NucleusCytoplasmCell MembraneTranslocates From The Nucleus To The Cytosol On Tcr StimulationWhile It Translocates Into The Nucleus In Ifn SignalingUsp12/Wdr20/Wdr48 Complex Is Localized Mainly To The Plasma Membrane |
| Alternative Antibody Names | Anti-Ubiquitin Carboxyl-Terminal Hydrolase 12 antibodyAnti-Deubiquitinating Enzyme 12 antibodyAnti-Ubiquitin Specific Peptidase 12 antibodyAnti-Ubiquitin Thioesterase 12 antibodyAnti-Ubiquitin-Hydrolyzing Enzyme 1 antibodyAnti-Ubiquitin-Specific-Processing Protease 12 antibodyAnti-USP12 antibodyAnti-UBH1 antibodyAnti-USP12L1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
