| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-USP10 (499-798) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
USP10 |
| Gene ID: |
9100 |
| Uniprot ID: |
UBP10_HUMAN |
| Immunogen Region: |
499-798 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 499-798 of human USP10 (NP_005144.2). |
| Immunogen Sequence: |
FEPTYIYRLLTVNKSSLSEK GRQEDAEEYLGFILNGLHEE MLNLKKLLSPSNEKLTISNG PKNHSVNEEEQEEQGEGSED EWEQVGPRNKTSVTRQADFV QTPITGIFGGHIRSVVYQQS SKESATLQPFFTLQLDIQSD KIRTVQDALESLVARESVQG YTTKTKQEVEISRRVTLEKL PPVLVLHLKRFVYEKTGGCQ KLIKNIEYPVDLEISKELLS PGVKNKNFKCHRTYRLFAV |
| Tissue Specificity | Widely expressed. |
| Post Translational Modifications | Phosphorylated by ATM following DNA damage, leading to stablization and translocation it to the nucleus. Ubiquitinated. Deubiquitinated by USP13. |
| Function | Hydrolase that can remove conjugated ubiquitin from target proteins such as p53/TP53, RPS2/us5, RPS3/us3, RPS10/eS10, BECN1, SNX3 and CFTR. Acts as an essential regulator of p53/TP53 stability: in unstressed cells, specifically deubiquitinates p53/TP53 in the cytoplasm, leading to counteract MDM2 action and stabilize p53/TP53. Following DNA damage, translocates to the nucleus and deubiquitinates p53/TP53, leading to regulate the p53/TP53-dependent DNA damage response. Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. In turn, PIK3C3/VPS34-containing complexes regulate USP10 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Does not deubiquitinate MDM2. Plays a key role in 40S ribosome subunit recycling when a ribosome has stalled during translation: acts both by inhibiting formation of stress granules, which store stalled translation pre-initiation complexes, and mediating deubiquitination of 40S ribosome subunits. Acts as a negative regulator of stress granules formation by lowering G3BP1 and G3BP2 valence, thereby preventing G3BP1 and G3BP2 ability to undergo liquid-liquid phase separation (LLPS) and assembly of stress granules. Promotes 40S ribosome subunit recycling following ribosome dissociation in response to ribosome stalling by mediating deubiquitination of 40S ribosomal proteins RPS2/us5, RPS3/us3 and RPS10/eS10, thereby preventing their degradation by the proteasome. Part of a ribosome quality control that takes place when ribosomes have stalled during translation initiation (iRQC): USP10 acts by removing monoubiquitination of RPS2/us5 and RPS3/us3, promoting 40S ribosomal subunit recycling. Deubiquitinates CFTR in early endosomes, enhancing its endocytic recycling. Involved in a TANK-dependent negative feedback response to attenuate NF-kappa-B activation via deubiquitinating IKBKG or TRAF6 in response to interleukin-1-beta (IL1B) stimulation or upon DNA damage. Deubiquitinates TBX21 leading to its stabilization. |
| Protein Name | Ubiquitin Carboxyl-Terminal Hydrolase 10Deubiquitinating Enzyme 10Ubiquitin Thioesterase 10Ubiquitin-Specific-Processing Protease 10 |
| Database Links | Reactome: R-HSA-5656169Reactome: R-HSA-5689880 |
| Cellular Localisation | CytoplasmNucleusEarly EndosomeCytoplasmic In Normal ConditionsAfter Dna DamageTranslocates To The Nucleus Following Phosphorylation By Atm |
| Alternative Antibody Names | Anti-Ubiquitin Carboxyl-Terminal Hydrolase 10 antibodyAnti-Deubiquitinating Enzyme 10 antibodyAnti-Ubiquitin Thioesterase 10 antibodyAnti-Ubiquitin-Specific-Processing Protease 10 antibodyAnti-USP10 antibodyAnti-KIAA0190 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
