• Confocal imaging of NIH/3T3 cells using USP10 Rabbit monoclonal antibody (STJ11102084, at dilution of 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
  • Confocal imaging of Hep G2 cells using USP10 Rabbit monoclonal antibody (STJ11102084, at dilution of 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
  • Western blot analysis of extracts of various cell lines, using USP10 Rabbit monoclonal antibody (STJ11102084) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-USP10 antibody (1-100) [S4MR] (STJ11102084)

SKU:
STJ11102084

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-USP10 (1-100) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: USP10
Gene ID: 9100
Uniprot ID: UBP10_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human USP10 (Q14694).
Immunogen Sequence: MALHSPQYIFGDFSPDEFNQ FFVTPRSSVELPPYSGTVLC GTQAVDKLPDGQEYQRIEFG VDEVIEPSDTLPRTPSYSIS STLNPQAPEFILGCTASKIT
Tissue Specificity Widely expressed.
Post Translational Modifications Phosphorylated by ATM following DNA damage, leading to stablization and translocation it to the nucleus. Ubiquitinated. Deubiquitinated by USP13.
Function Hydrolase that can remove conjugated ubiquitin from target proteins such as p53/TP53, RPS2/us5, RPS3/us3, RPS10/eS10, BECN1, SNX3 and CFTR. Acts as an essential regulator of p53/TP53 stability: in unstressed cells, specifically deubiquitinates p53/TP53 in the cytoplasm, leading to counteract MDM2 action and stabilize p53/TP53. Following DNA damage, translocates to the nucleus and deubiquitinates p53/TP53, leading to regulate the p53/TP53-dependent DNA damage response. Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. In turn, PIK3C3/VPS34-containing complexes regulate USP10 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Does not deubiquitinate MDM2. Plays a key role in 40S ribosome subunit recycling when a ribosome has stalled during translation: acts both by inhibiting formation of stress granules, which store stalled translation pre-initiation complexes, and mediating deubiquitination of 40S ribosome subunits. Acts as a negative regulator of stress granules formation by lowering G3BP1 and G3BP2 valence, thereby preventing G3BP1 and G3BP2 ability to undergo liquid-liquid phase separation (LLPS) and assembly of stress granules. Promotes 40S ribosome subunit recycling following ribosome dissociation in response to ribosome stalling by mediating deubiquitination of 40S ribosomal proteins RPS2/us5, RPS3/us3 and RPS10/eS10, thereby preventing their degradation by the proteasome. Part of a ribosome quality control that takes place when ribosomes have stalled during translation initiation (iRQC): USP10 acts by removing monoubiquitination of RPS2/us5 and RPS3/us3, promoting 40S ribosomal subunit recycling. Deubiquitinates CFTR in early endosomes, enhancing its endocytic recycling. Involved in a TANK-dependent negative feedback response to attenuate NF-kappa-B activation via deubiquitinating IKBKG or TRAF6 in response to interleukin-1-beta (IL1B) stimulation or upon DNA damage. Deubiquitinates TBX21 leading to its stabilization.
Protein Name Ubiquitin Carboxyl-Terminal Hydrolase 10
Deubiquitinating Enzyme 10
Ubiquitin Thioesterase 10
Ubiquitin-Specific-Processing Protease 10
Database Links Reactome: R-HSA-5656169
Reactome: R-HSA-5689880
Cellular Localisation Cytoplasm
Nucleus
Early Endosome
Cytoplasmic In Normal Conditions
After Dna Damage
Translocates To The Nucleus Following Phosphorylation By Atm
Alternative Antibody Names Anti-Ubiquitin Carboxyl-Terminal Hydrolase 10 antibody
Anti-Deubiquitinating Enzyme 10 antibody
Anti-Ubiquitin Thioesterase 10 antibody
Anti-Ubiquitin-Specific-Processing Protease 10 antibody
Anti-USP10 antibody
Anti-KIAA0190 antibody

Information sourced from Uniprot.org

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