| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-USP1 (1-280) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
USP1 |
| Gene ID: |
7398 |
| Uniprot ID: |
UBP1_HUMAN |
| Immunogen Region: |
1-280 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human USP1 (NP_003359.3). |
| Immunogen Sequence: |
MPGVIPSESNGLSRGSPSKK NRLSLKFFQKKETKRALDFT DSQENEEKASEYRASEIDQV VPAAQSSPINCEKRENLLPF VGLNNLGNTCYLNSILQVLY FCPGFKSGVKHLFNIISRKK EALKDEANQKDKGNCKEDSL ASYELICSLQSLIISVEQLQ ASFLLNPEKYTDELATQPRR LLNTLRELNPMYEGYLQHDA QEVLQCILGNIQETCQLLKK EEVKNVAELPTKVEEIPHP |
| Post Translational Modifications | Autocatalytic cleavage of USP1 following UV irradiation inactivates it, leading to an increase in ubiquitinated PCNA, recruitment of POLH and translesion synthesis. Ubiquitin carboxyl-terminal hydrolase 1, N-terminal fragment: Ubiquitinated by the CRL2(KLHDC2) complex following autocatalytic cleavage, leading to its degradation: the CRL2(KLHDC2) complex recognizes the diglycine (Gly-Gly) at the C-terminus. |
| Function | Negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2. Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA. Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity. |
| Protein Name | Ubiquitin Carboxyl-Terminal Hydrolase 1Deubiquitinating Enzyme 1HubpUbiquitin Thioesterase 1Ubiquitin-Specific-Processing Protease 1 Cleaved Into - Ubiquitin Carboxyl-Terminal Hydrolase 1 - N-Terminal Fragment |
| Database Links | Reactome: R-HSA-110314Reactome: R-HSA-6783310 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Ubiquitin Carboxyl-Terminal Hydrolase 1 antibodyAnti-Deubiquitinating Enzyme 1 antibodyAnti-Hubp antibodyAnti-Ubiquitin Thioesterase 1 antibodyAnti-Ubiquitin-Specific-Processing Protease 1 Cleaved Into - Ubiquitin Carboxyl-Terminal Hydrolase 1 - N-Terminal Fragment antibodyAnti-USP1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
