• Western blot analysis of extracts of various cell lines, using USO1 antibody (STJ118519) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunofluorescence analysis of HeLa cells using USO1 antibody (STJ118519) at dilution of 1:200 (60x lens). Blue: DAPI for nuclear staining.
  • Confocal immunofluorescence analysis of Hela cells using USO1 antibody (STJ118519) at dilution of 1:200. Blue: DAPI for nuclear staining.
  • Confocal immunofluorescence analysis of HeLa cells using USO1 Polyclonal Antibody (STJ118519) at dilution of 1:50. Blue: DAPI for nuclear staining.

Anti-USO1 antibody (663-962) (STJ118519)

SKU:
STJ118519

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-USO1 (663-962) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: USO1
Gene ID: 8615
Uniprot ID: USO1_HUMAN
Immunogen Region: 663-962
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 663-962 of human USO1 (NP_003706.2).
Immunogen Sequence: DLQLEELRQQVSTLKCQNEQ LQTAVTQQVSQIQQHKDQYN LLKIQLGKDNQHQGSYSEGA QMNGIQPEEIGRLREEIEEL KRNQELLQSQLTEKDSMIEN MKSSQTSGTNEQSSAIVSAR DSEQVAELKQELATLKSQLN SQSVEITKLQTEKQELLQKT EAFAKSVEVQGETETIIATK TTDVEGRLSALLQETKELKN EIKALSEERTAIKEQLDSSN STIAILQTEKDKLELEITD
Post Translational Modifications Phosphorylated in a cell cycle-specific manner.phosphorylated in interphase but not in mitotic cells. Dephosphorylated protein associates with the Golgi membrane.phosphorylation promotes dissociation.
Function General vesicular transport factor required for intercisternal transport in the Golgi stack.it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity.
Protein Name General Vesicular Transport Factor P115
Protein Uso1 Homolog
Transcytosis-Associated Protein
Tap
Vesicle-Docking Protein
Database Links Reactome: R-HSA-162658
Reactome: R-HSA-204005
Reactome: R-HSA-6807878
Cellular Localisation Cytoplasm
Cytosol
Golgi Apparatus Membrane
Peripheral Membrane Protein
Recycles Between The Cytosol And The Golgi Apparatus During Interphase
During Interphase
The Phosphorylated Form Is Found Exclusively In Cytosol
The Unphosphorylated Form Is Associated With Golgi Apparatus Membranes
Alternative Antibody Names Anti-General Vesicular Transport Factor P115 antibody
Anti-Protein Uso1 Homolog antibody
Anti-Transcytosis-Associated Protein antibody
Anti-Tap antibody
Anti-Vesicle-Docking Protein antibody
Anti-USO1 antibody
Anti-VDP antibody

Information sourced from Uniprot.org

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