• Western blot analysis of extracts of various cell lines, using UQCC2 antibody (STJ112027) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Immunohistochemistry analysis of paraffin-embedded mouse heart using UQCC2 antibody (STJ112027) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat spleen using UQCC2 antibody (STJ112027) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded rat heart using UQCC2 antibody (STJ112027) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat heart using UQCC2 antibody (STJ112027) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded rat spleen using UQCC2 antibody (STJ112027) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse heart using UQCC2 antibody (STJ112027) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of various cell lines, using UQCC2 antibody (STJ112027) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-UQCC2 antibody (14-126) (STJ112027)

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STJ112027

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-UQCC2 (14-126) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: UQCC2
Gene ID: 84300
Uniprot ID: UQCC2_HUMAN
Immunogen Region: 14-126
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 14-126 of human UQCC2 (NP_115716.1).
Immunogen Sequence: EEWPVDETKRGRDLGAYLRQ RVAQAFREGENTQVAEPEAC DQMYESLARLHSNYYKHKYP RPRDTSFSGLSLEEYKLILS TDTLEELKEIDKGMWKKLQE KFAPKGPEEDHKA
Tissue Specificity Pancreas, skeletal muscle, kidney, liver and heart.
Function Required for the assembly of the ubiquinol-cytochrome c reductase complex (mitochondrial respiratory chain complex III or cytochrome b-c1 complex). Plays a role in the modulation of respiratory chain activities such as oxygen consumption and ATP production and via its modulation of the respiratory chain activity can regulate skeletal muscle differentiation and insulin secretion by pancreatic beta-cells. Involved in cytochrome b translation and/or stability.
Protein Name Ubiquinol-Cytochrome C Reductase Complex Assembly Factor 2
Breast Cancer-Associated Protein Sga-81m
Mitochondrial Nucleoid Factor 1
Mitochondrial Protein M19
Cellular Localisation Mitochondrion Matrix
Mitochondrion Nucleoid
Mitochondrion
Mitochondrion Intermembrane Space
Mitochondrion Inner Membrane
Predominantly Expressed In The Mitochondrial Inner Membrane
Alternative Antibody Names Anti-Ubiquinol-Cytochrome C Reductase Complex Assembly Factor 2 antibody
Anti-Breast Cancer-Associated Protein Sga-81m antibody
Anti-Mitochondrial Nucleoid Factor 1 antibody
Anti-Mitochondrial Protein M19 antibody
Anti-UQCC2 antibody
Anti-C6orf125 antibody
Anti-MNF1 antibody

Information sourced from Uniprot.org

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