• Western blot analysis of lysates from 293T cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-UPF3A antibody (190-240 aa) (STJ194832)

SKU:
STJ194832

Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Regulator of nonsense transcripts 3A (190-240 aa) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC-P 1:50-300
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: UPF3A
Gene ID: 65110
Uniprot ID: REN3A_HUMAN
Immunogen Region: 190-240 aa
Specificity: This antibody detects endogenous levels of REN3A at Human
Immunogen: Synthesized peptide derived from the human REN3A at the amino acid range 190-240
Function Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex (EJC) and serving as link between the EJC core and NMD machinery. Recruits UPF2 at the cytoplasmic side of the nuclear envelope and the subsequent formation of an UPF1-UPF2-UPF3 surveillance complex (including UPF1 bound to release factors at the stalled ribosome) is believed to activate NMD. However, UPF3A is shown to be only marginally active in NMD as compared to UPF3B. Binds spliced mRNA upstream of exon-exon junctions. In vitro, weakly stimulates translation.
Protein Name Regulator Of Nonsense Transcripts 3a
Nonsense Mrna Reducing Factor 3a
Up-Frameshift Suppressor 3 Homolog A
Hupf3
Database Links Reactome: R-HSA-9010553
Reactome: R-HSA-975957 Q9H1J1-2
Cellular Localisation Nucleus
Cytoplasm
Shuttling Between The Nucleus And The Cytoplasm
Alternative Antibody Names Anti-Regulator Of Nonsense Transcripts 3a antibody
Anti-Nonsense Mrna Reducing Factor 3a antibody
Anti-Up-Frameshift Suppressor 3 Homolog A antibody
Anti-Hupf3 antibody
Anti-UPF3A antibody
Anti-RENT3A antibody
Anti-UPF3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance