• Immunofluorescence analysis of L929 cells using UNC13B Rabbit polyclonal antibody (STJ118225) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of UNC13B in paraffin-embedded Human liver using UNC13B Rabbit polyclonal antibody (STJ118225) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using UNC13B Rabbit polyclonal antibody (STJ118225) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-UNC13B antibody (1-350) (STJ118225)

SKU:
STJ118225

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-UNC13B (1-350) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: UNC13B
Gene ID: 10497
Uniprot ID: UN13B_HUMAN
Immunogen Region: 1-350
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human UNC13B (NP_006368.3).
Immunogen Sequence: MSLLCVRVKRAKFQGSPDKF NTYVTLKVQNVKSTTVAVRG DQPSWEQDFMFEISRLDLGL SVEVWNKGLIWDTMVGTVWI ALKTIRQSDEEGPGEWSTLE AETLMKDDEICGTRNPTPHK ILLDTRFELPFDIPEEEARY WTYKWEQINALGADNEYSSQ EESQRKPLPTAAAQCSFEDP DSAVDDRDSDYRSETSNSFP PPYHTASQPNASVHQFPVPV RSPQQLLLQGSSRDSCNDS
Tissue Specificity Expressed in kidney cortical epithelial cells and brain.
Function Plays a role in vesicle maturation during exocytosis as a target of the diacylglycerol second messenger pathway. Is involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and participates in the activity-depending refilling of readily releasable vesicle pool (RRP). Essential for synaptic vesicle maturation in a subset of excitatory/glutamatergic but not inhibitory/GABA-mediated synapses. In collaboration with UNC13A, facilitates neuronal dense core vesicles fusion as well as controls the location and efficiency of their synaptic release.
Protein Name Protein Unc-13 Homolog B
Munc13-2
Munc13
Database Links Reactome: R-HSA-181429
Reactome: R-HSA-181430
Reactome: R-HSA-210500
Reactome: R-HSA-212676
Reactome: R-HSA-264642
Cellular Localisation Cytoplasm
Membrane
Peripheral Membrane Protein
Cell Membrane
Synapse
Localized To Synapses
Translocated To The Plasma Membrane In Response To Phorbol Ester Binding
Alternative Antibody Names Anti-Protein Unc-13 Homolog B antibody
Anti-Munc13-2 antibody
Anti-Munc13 antibody
Anti-UNC13B antibody
Anti-UNC13 antibody

Information sourced from Uniprot.org

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