| Host: | Rabbit |
| Applications: | ELISA/IHC/IP/WB |
| Reactivity: | Bovine/Human/Monkey/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-ULK3 (452-472) is suitable for use in ELISA, Immunohistochemistry, Immunoprecipitation and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Biotin |
| Isotype: | IgG |
| Formulation: | Contains Tris, HCl/Glycine buffer pH 7.4-7.8, 30% Glycerol and 0.5% BSA, along with cryo-protective agents, Hepes, and long-term preservatives (0.02% Sodium Azide). |
| Purification: | Affinity Purified |
| Concentration: | 0.65 µg/µl |
| Dilution Range: | WB: 1:500 DB: 1:10, 000 ELISA: 1:10, 000 IP: 1:200 IHC: 1:50-1:100 |
| Storage Instruction: | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: | Ulk3 |
| Gene ID: | 691171 |
| Uniprot ID: | ULK3_RAT |
| Immunogen Region: | 452-472 |
| Immunogen: | Synthetic peptide corresponding to a region within C terminal amino acids 452-472 of Mouse ULK3, coupled to a carrier protein. |
| Post Translational Modifications | Autophosphorylated. Autophosphorylation is blocked by interaction with SUFU. |
| Function | Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy. |
| Protein Name | Serine/Threonine-Protein Kinase Ulk3 Unc-51-Like Kinase 3 |
| Database Links | Reactome: R-RNO-5632684 |
| Cellular Localisation | Cytoplasm Localizes To Pre-Autophagosomal Structure During Cellular Senescence |
| Alternative Antibody Names | Anti-Serine/Threonine-Protein Kinase Ulk3 antibody Anti-Unc-51-Like Kinase 3 antibody Anti-Ulk3 antibody |
Information sourced from Uniprot.org