Anti-UIMC1 antibody (1-150) (STJ29387)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal UIMC1 (1-150) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | UIMC1 |
| Gene ID | 51720 |
| Uniprot ID | UIMC1_HUMAN |
| Immunogen Region | 1-150 |
| Immunogen Sequence | MPRRKKKVKEVSESRNLEKK DVETTSSVSVKRKRRLEDAF IVISDSDGEEPKEENGLQKT KTKQSNRAKCLAKRKIAQMT EEEQFALALKMSEQEAREVN SQEEEEEELLRKAIAESLNS CRPSDASATRSRPLATGPSS QSHQEKTTDS |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human UIMC1 (NP_057374.3). |
Additional Info
| Tissue Specificity | Expressed in testis, ovary, thymus and heart. Expressed in germ cells of the testis. |
| Post Translational Modifications | Sumoylated. Phosphorylated upon DNA damage by ATM or ATR. |
| Function | Ubiquitin-binding protein. Specifically recognizes and binds 'Lys-63'-linked ubiquitin. Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. Also weakly binds monoubiquitin but with much less affinity than 'Lys-63'-linked ubiquitin. May interact with monoubiquitinated histones H2A and H2B.the relevance of such results is however unclear in vivo. Does not bind Lys-48'-linked ubiquitin. May indirectly act as a transcriptional repressor by inhibiting the interaction of NR6A1 with the corepressor NCOR1. |
| Protein Name | Brca1-A Complex Subunit Rap80Receptor-Associated Protein 80Retinoid X Receptor-Interacting Protein 110Ubiquitin Interaction Motif-Containing Protein 1 |
| Database Links | Reactome: R-HSA-5689901Reactome: R-HSA-5693565Reactome: R-HSA-5693571Reactome: R-HSA-5693607Reactome: R-HSA-69473 |
| Cellular Localisation | NucleusLocalizes At Sites Of Dna Damage At Double-Strand Breaks (Dsbs) |
| Alternative Antibody Names | Anti-Brca1-A Complex Subunit Rap80 antibodyAnti-Receptor-Associated Protein 80 antibodyAnti-Retinoid X Receptor-Interacting Protein 110 antibodyAnti-Ubiquitin Interaction Motif-Containing Protein 1 antibodyAnti-UIMC1 antibodyAnti-RAP80 antibodyAnti-RXRIP110 antibody |
Information sourced from Uniprot.org