Host: |
Rabbit |
Applications: |
IHC/WB |
Reactivity: |
Mouse/Rat |
Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description: |
Rabbit polyclonal antibody anti-UHRF1 (2-50 aa) is suitable for use in Immunohistochemistry and Western Blot research applications. |
Clonality: |
Polyclonal |
Conjugation: |
Unconjugated |
Isotype: |
IgG |
Formulation: |
Lyophilised |
Purification: |
IgG purified |
Dilution Range: |
IHC, WB. A dilution of 1:300 to 1:2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
Storage Instruction: |
Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
Gene Symbol: |
Uhrf1 |
Gene ID: |
18140 |
Uniprot ID: |
UHRF1_MOUSE |
Immunogen Region: |
2-50 aa |
Specificity: |
Specific for UHRF1. |
Immunogen: |
A synthetic peptide from aa region 2-50 of mouse UHRF1 conjugated to an immunogenic carrier protein was used as the antigen. |
Post Translational Modifications | Phosphorylation at Ser-303 of the linker region decreases the binding to H3K9me3. Phosphorylation at Ser-639 by CDK1 during M phase impairs interaction with USP7, preventing deubiquitination and leading to degradation by the proteasome. Ubiquitinated.which leads to proteasomal degradation. Autoubiquitinated.interaction with USP7 leads to deubiquitination and prevents degradation. Ubiquitination and degradation takes place during M phase, when phosphorylation at Ser-639 prevents interaction with USP7 and subsequent deubiquitination. Polyubiquitination may be stimulated by DNA damage. |
Function | Multidomain protein that acts as a key epigenetic regulator by bridging DNA methylation and chromatin modification. Specifically recognizes and binds hemimethylated DNA at replication forks via its YDG domain and recruits DNMT1 methyltransferase to ensure faithful propagation of the DNA methylation patterns through DNA replication. In addition to its role in maintenance of DNA methylation, also plays a key role in chromatin modification: through its tudor-like regions and PHD-type zinc fingers, specifically recognizes and binds histone H3 trimethylated at 'Lys-9' (H3K9me3) and unmethylated at 'Arg-2' (H3R2me0), respectively, and recruits chromatin proteins. Enriched in pericentric heterochromatin where it recruits different chromatin modifiers required for this chromatin replication. Also localizes to euchromatic regions where it negatively regulates transcription possibly by impacting DNA methylation and histone modifications. Has E3 ubiquitin-protein ligase activity by mediating the ubiquitination of target proteins such as histone H3 and PML. It is still unclear how E3 ubiquitin-protein ligase activity is related to its role in chromatin in vivo. Plays a role in DNA repair by cooperating with UHRF2 to ensure recruitment of FANCD2 to interstrand cross-links (ICLs) leading to FANCD2 activation. Plays a pivotal role in the establishment of correct spindle architecture by catalyzing the 'Lys-63'-linked ubiquitination of KIF11, thereby controlling KIF11 localization on the spindle. |
Protein Name | E3 Ubiquitin-Protein Ligase Uhrf1Nuclear Protein 95Nuclear Zinc Finger Protein Np95Ring-Type E3 Ubiquitin Transferase Uhrf1Ubiquitin-Like Phd And Ring Finger Domain-Containing Protein 1Muhrf1Ubiquitin-Like-Containing Phd And Ring Finger Domains Protein 1 |
Cellular Localisation | NucleusAssociatedThrough The Ydg Domain (Also Called Sra Domain)With Replicating Dna From Early To Late S PhaseIncluding At Replicating Pericentric HeterochromatinAlso Localizes To Euchromatic RegionsIn Non-S-Phase CellsHomogenously Distributed Through The Nucleus |
Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Uhrf1 antibodyAnti-Nuclear Protein 95 antibodyAnti-Nuclear Zinc Finger Protein Np95 antibodyAnti-Ring-Type E3 Ubiquitin Transferase Uhrf1 antibodyAnti-Ubiquitin-Like Phd And Ring Finger Domain-Containing Protein 1 antibodyAnti-Muhrf1 antibodyAnti-Ubiquitin-Like-Containing Phd And Ring Finger Domains Protein 1 antibodyAnti-Uhrf1 antibodyAnti-Np95 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance