• Western blot analysis of extracts of various cell lines, using UFD1L antibody (STJ11104143) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-UFD1 antibody (1-100) [S4143RM] (STJ11104143)

SKU:
STJ11104143

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Ubiquitin Recognition Factor In Er-Associated Degradation Protein 1 (1-100) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S4143RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: UFD1
Gene ID: 7353
Uniprot ID: UFD1_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human UFD1L (Q92890).
Immunogen Sequence: MFSFNMFDHPIPRVFQNRFS TQYRCFSVSMLAGPNDRSDV EKGGKIIMPPSALDQLSRLN ITYPMLFKLTNKNSDRMTHC GVLEFVADEGICYLPHWMMQ
Tissue Specificity Found in adult heart, skeletal muscle and pancreas, and in fetal liver and kidney.
Function Essential component of the ubiquitin-dependent proteolytic pathway which degrades ubiquitin fusion proteins. The ternary complex containing UFD1, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. It may be involved in the development of some ectoderm-derived structures. Acts as a negative regulator of type I interferon production via the complex formed with VCP and NPLOC4, which binds to RIGI and recruits RNF125 to promote ubiquitination and degradation of RIGI.
Protein Name Ubiquitin Recognition Factor In Er-Associated Degradation Protein 1
Ubiquitin Fusion Degradation Protein 1
Ub Fusion Protein 1
Database Links Reactome: R-HSA-110320
Reactome: R-HSA-5689880
Reactome: R-HSA-8951664
Reactome: R-HSA-9755511
Cellular Localisation Nucleus
Cytoplasm
Cytosol
Alternative Antibody Names Anti-Ubiquitin Recognition Factor In Er-Associated Degradation Protein 1 antibody
Anti-Ubiquitin Fusion Degradation Protein 1 antibody
Anti-Ub Fusion Protein 1 antibody
Anti-UFD1 antibody
Anti-UFD1L antibody

Information sourced from Uniprot.org

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