• Western blot analysis of various lysates, using UFC1 Rabbit monoclonal antibody (STJ11104205) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of various lysates, using UFC1 Rabbit monoclonal antibody (STJ11104205) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-UFC1 antibody (1-130) [S4205RM] (STJ11104205)

SKU:
STJ11104205

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Ubiquitin-Fold Modifier-Conjugating Enzyme 1 (1-130) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S4205RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: UFC1
Gene ID: 51506
Uniprot ID: UFC1_HUMAN
Immunogen Region: 1-130
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of Human UFC1 (NP_057490.2).
Immunogen Sequence: MADEATRRVVSEIPVLKTNA GPRDRELWVQRLKEEYQSLI RYVENNKNADNDWFRLESNK EGTRWFGKCWYIHDLLKYEF DIEFDIPITYPTTAPEIAVP ELDGKTAKMYRGGKICLTDH FKPLWARNVP
Function E1-like enzyme which specifically catalyzes the second step in ufmylation. Accepts the ubiquitin-like modifier UFM1 from the E1 enzyme UBA5 and forms an intermediate with UFM1 via a thioester linkage. Ufmylation is involved in reticulophagy (also called ER-phagy) induced in response to endoplasmic reticulum stress.
Protein Name Ubiquitin-Fold Modifier-Conjugating Enzyme 1
Ufm1-Conjugating Enzyme 1
Alternative Antibody Names Anti-Ubiquitin-Fold Modifier-Conjugating Enzyme 1 antibody
Anti-Ufm1-Conjugating Enzyme 1 antibody
Anti-UFC1 antibody
Anti-CGI-126 antibody
Anti-HSPC155 antibody

Information sourced from Uniprot.org

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