• Western blot analysis of various lysates using UCP2 Rabbit polyclonal antibody (STJ26031) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-UCP2 antibody (112-211) (STJ26031)

SKU:
STJ26031

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-UCP2 (112-211) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: UCP2
Gene ID: 7351
Uniprot ID: UCP2_HUMAN
Immunogen Region: 112-211
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 112-211 of human UCP2 (NP_003346.2).
Immunogen Sequence: EHASIGSRLLAGSTTGALAV AVAQPTDVVKVRFQAQARAG GGRRYQSTVNAYKTIAREEG FRGLWKGTSPNVARNAIVNC AELVTYDLIKDALLKANLMT
Tissue Specificity Widely expressed in adult human tissues, including tissues rich in macrophages. Most expressed in white adipose tissue and skeletal muscle.
Function Antiporter that exports dicarboxylate intermediates of the Krebs cycle in exchange for phosphate plus a proton across the inner membrane of mitochondria, a process driven by mitochondrial motive force with an overall impact on glycolysis, glutaminolysis and glutathione-dependent redox balance. Continuous export of oxaloacetate and related four-carbon dicarboxylates from mitochondrial matrix into the cytosol negatively regulates the oxidation of acetyl-CoA substrates via the Krebs cycle, lowering the ATP/ADP ratio and reactive oxygen species (ROS) production. Proton transporter activity is debated, but if it occurs it may mediate inducible proton re-entry into the mitochondrial matrix affecting ATP turnover as a protection mechanism against oxidative stress. Proton re-entry may be coupled to metabolite transport to allow for proton flux switching and optimal ATP turnover. Regulates the use of glucose as a source of energy. Required for glucose-induced DRP1-dependent mitochondrial fission and neuron activation in the ventromedial nucleus of the hypothalamus (VMH). This mitochondrial adaptation mechanism modulates the VMH pool of glucose-excited neurons with an impact on systemic glucose homeostasis. Regulates ROS levels and metabolic reprogramming of macrophages during the resolution phase of inflammation. Attenuates ROS production in response to IL33 to preserve the integrity of the Krebs cycle required for persistent production of itaconate and subsequent GATA3-dependent differentiation of inflammation-resolving alternatively activated macrophages. Can unidirectionally transport anions including L-malate, L-aspartate, phosphate and chloride ions. Does not mediate adaptive thermogenesis.
Protein Name Dicarboxylate Carrier Slc25a8
Mitochondrial Uncoupling Protein 2
Ucp 2
Solute Carrier Family 25 Member 8
Ucph
Database Links Reactome: R-HSA-167826
Reactome: R-HSA-167827
Cellular Localisation Mitochondrion Inner Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Dicarboxylate Carrier Slc25a8 antibody
Anti-Mitochondrial Uncoupling Protein 2 antibody
Anti-Ucp 2 antibody
Anti-Solute Carrier Family 25 Member 8 antibody
Anti-Ucph antibody
Anti-UCP2 antibody
Anti-SLC25A8 antibody

Information sourced from Uniprot.org

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