• Immunofluorescence analysis of rat brown adipose cells using UCP1+UCP3 Rabbit monoclonal antibody (STJ11103032) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse brown adipose using UCP1+UCP3 Rabbit monoclonal antibody (STJ11103032) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brown adipose using UCP1+UCP3 Rabbit monoclonal antibody (STJ11103032) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using UCP1+UCP3 Rabbit monoclonal antibody (STJ11103032) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-UCP1+UCP3 antibody (1-307) [S2MR] (STJ11103032)

SKU:
STJ11103032

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-UCP1+UCP3 (1-307) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S2MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 1-307
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-307 of mouse UCP1+UCP3 (P12242).
Immunogen Sequence: MVNPTTSEVQPTMGVKIFSA GVSACLADIITFPLDTAKVR LQIQGEGQASSTIRYKGVLG TITTLAKTEGLPKLYSGLPA GIQRQISFASLRIGLYDSVQ EYFSSGRETPASLGNKISAG LMTGGVAVFIGQPTEVVKVR MQAQSHLHGIKPRYTGTYNA YRVIATTESLSTLWKGTTPN LMRNVIINCTELVTYDLMKG ALVNNKILADDVPCHLLSAL VAGFCTTLLASPVDVVKTR
Background MAD1L1 is a component of the mitotic spindle-assembly checkpoint that prevents the onset of anaphase until all chromosome are properly aligned at the metaphase plate. MAD1L1 functions as a homodimer and interacts with MAD2L1. MAD1L1 may play a role in cell cycle control and tumor suppression. Alternative splicing results in multiple transcript variants.

Information sourced from Uniprot.org

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