• Immunohistochemistry analysis of UCKL1 in paraffin-embedded Human breast cancer using UCKL1 Rabbit polyclonal antibody (STJ118296) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using UCKL1 Rabbit polyclonal antibody (STJ118296) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-UCKL1 antibody (1-200) (STJ118296)

SKU:
STJ118296

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-UCKL1 (1-200) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: UCKL1
Gene ID: 54963
Uniprot ID: UCKL1_HUMAN
Immunogen Region: 1-200
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human UCKL1 (NP_060329.2).
Immunogen Sequence: MAAPPARADADPSPTSPPTA RDTPGRQAEKSETACEDRSN AESLDRLLPPVGTGRSPRKR TTSQCKSEPPLLRTSKRTIY TAGRPPWYNEHGTQSKEAFA IGLGGGSASGKTTVARMIIE ALDVPWVVLLSMDSFYKVLT EQQQEQAAHNNFNFDHPDAF DFDLIISTLKKLKQGKSVKV PIYDFTTHSRKKDWKTLYGA
Tissue Specificity Ubiquitous.
Post Translational Modifications Ubiquitinated by RNF19B.which induces proteasomal degradation.
Function May contribute to UTP accumulation needed for blast transformation and proliferation.
Protein Name Uridine-Cytidine Kinase-Like 1
Database Links Reactome: R-HSA-73614
Cellular Localisation Cytoplasm
Nucleus
Ebna3 Induces Isoform 1 Translocation To The Nucleus
Whereas It Does Change Isoform 3 Location
Alternative Antibody Names Anti-Uridine-Cytidine Kinase-Like 1 antibody
Anti-UCKL1 antibody
Anti-URKL1 antibody
Anti-F538 antibody

Information sourced from Uniprot.org

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