• Western blot analysis of extracts of various cell lines, using UBTF antibody (STJ111889) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Western blot analysis of extracts of Rat kidney, using UBTF antibody (STJ111889) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.
  • Western blot analysis of extracts of Rat kidney, using UBTF antibody (STJ111889) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.
  • Western blot analysis of extracts of various cell lines, using UBTF antibody (STJ111889) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-UBTF antibody (200-440) (STJ111889)

SKU:
STJ111889

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-UBTF (200-440) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: UBTF
Gene ID: 7343
Uniprot ID: UBF1_HUMAN
Immunogen Region: 200-440
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 200-440 of human UBTF (NP_055048.1).
Immunogen Sequence: KTPQQLWYTHEKKVYLKVRP DATTKEVKDSLGKQWSQLSD KKRLKWIHKALEQRKEYEEI MRDYIQKHPELNISEEGITK STLTKAERQLKDKFDGRPTK PPPNSYSLYCAELMANMKDV PSTERMVLCSQQWKLLSQKE KDAYHKKCDQKKKDYEVELL RFLESLPEEEQQRVLGEEKM LNINKKQATSPASKKPAQEG GKGGSEKPKRPVSAMFIFSE EKRRQLQEERPELSESELT
Post Translational Modifications Phosphorylated and activated by PIK3CA.
Function Recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element.
Protein Name Nucleolar Transcription Factor 1
Autoantigen Nor-90
Upstream-Binding Factor 1
Ubf-1
Database Links Reactome: R-HSA-427413
Reactome: R-HSA-73728
Reactome: R-HSA-73762
Reactome: R-HSA-73772
Reactome: R-HSA-73863
Cellular Localisation Nucleus
Nucleolus
Alternative Antibody Names Anti-Nucleolar Transcription Factor 1 antibody
Anti-Autoantigen Nor-90 antibody
Anti-Upstream-Binding Factor 1 antibody
Anti-Ubf-1 antibody
Anti-UBTF antibody
Anti-UBF antibody
Anti-UBF1 antibody

Information sourced from Uniprot.org

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