• Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g UBL4A antibody (STJ26569). Western blot was performed from the immunoprecipitate using UBL4A antibody (STJ26569) at a dilution of 1:1000.
  • Western blot analysis of various lysates using UBL4A Rabbit polyclonal antibody (STJ26569) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-UBL4A antibody (1-157) (STJ26569)

SKU:
STJ26569

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IP/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:200-1:1000
IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: UBL4A
Gene ID: 8266
Uniprot ID: UBL4A_HUMAN
Immunogen Region: 1-157
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-157 of human UBL4A (NP_055050.1).
Immunogen Sequence: MQLTVKALQGRECSLQVPED ELVSTLKQLVSEKLNVPVRQ QRLLFKGKALADGKRLSDYS IGPNSKLNLVVKPLEKVLLE EGEAQRLADSPPPQVWQLIS KVLARHFSAADASRVLEQLQ RDYERSLSRLTLDDIERLAS RFLHPEVTETMEKGFSK
Post Translational Modifications Polyubiquitinated. Ubiquitination by AMFR and deubiquitination by USP13 may regulate the interaction between the BAG6/BAT3 complex and SGTA and therefore may regulate client proteins fate.
Function As part of a cytosolic protein quality control complex, the BAG6/BAT3 complex, maintains misfolded and hydrophobic patches-containing proteins in a soluble state and participates in their proper delivery to the endoplasmic reticulum or alternatively can promote their sorting to the proteasome where they undergo degradation. The BAG6/BAT3 complex is involved in the post-translational delivery of tail-anchored/type II transmembrane proteins to the endoplasmic reticulum membrane. Recruited to ribosomes, it interacts with the transmembrane region of newly synthesized tail-anchored proteins and together with SGTA and ASNA1 mediates their delivery to the endoplasmic reticulum. Client proteins that cannot be properly delivered to the endoplasmic reticulum are ubiquitinated and sorted to the proteasome. Similarly, the BAG6/BAT3 complex also functions as a sorting platform for proteins of the secretory pathway that are mislocalized to the cytosol either delivering them to the proteasome for degradation or to the endoplasmic reticulum. The BAG6/BAT3 complex also plays a role in the endoplasmic reticulum-associated degradation (ERAD), a quality control mechanism that eliminates unwanted proteins of the endoplasmic reticulum through their retrotranslocation to the cytosol and their targeting to the proteasome. It maintains these retrotranslocated proteins in an unfolded yet soluble state condition in the cytosol to ensure their proper delivery to the proteasome.
Protein Name Ubiquitin-Like Protein 4a
Ubiquitin-Like Protein Gdx
Database Links Reactome: R-HSA-9609523
Cellular Localisation Cytoplasm
Cytosol
Nucleus
Alternative Antibody Names Anti-Ubiquitin-Like Protein 4a antibody
Anti-Ubiquitin-Like Protein Gdx antibody
Anti-UBL4A antibody
Anti-DXS254E antibody
Anti-GDX antibody
Anti-UBL4 antibody

Information sourced from Uniprot.org

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