• Western blot analysis of HuvEc cells using UBE3C Polyclonal Antibody. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).
  • Western blot analysis of various cells using UBE3C Polyclonal Antibody. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-UBE3C antibody (Internal) (STJ96167)

SKU:
STJ96167

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Ubiquitin-protein ligase E3C (Internal) is suitable for use in Western Blot and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: UBE3C
Gene ID: 9690
Uniprot ID: UBE3C_HUMAN
Immunogen Region: Internal
Specificity: UBE3C Polyclonal Antibody detects endogenous levels of UBE3C protein.
Immunogen: Synthesized peptide derived from the Internal region of human UBE3C.
Post Translational Modifications Autoubiquitinated.promoting its own degradation.
Function E3 ubiquitin-protein ligase that specifically catalyzes 'Lys-29'- and 'Lys-48'-linked polyubiquitin chains. Accepts ubiquitin from the E2 ubiquitin-conjugating enzyme UBE2D1 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Associates with the proteasome and promotes elongation of ubiquitin chains on substrates bound to the 26S proteasome. Also catalyzes 'Lys-29'- and 'Lys-48'-linked ubiquitination of 26S proteasome subunit ADRM1/RPN13 in response to proteotoxic stress, impairing the ability of the proteasome to bind and degrade ubiquitin-conjugated proteins. Acts as a negative regulator of autophagy by mediating 'Lys-29'- and 'Lys-48'-linked ubiquitination of PIK3C3/VPS34, promoting its degradation. Can assemble unanchored poly-ubiquitin chains in either 'Lys-29'- or 'Lys-48'-linked polyubiquitin chains.with some preference for 'Lys-48' linkages. Acts as a negative regulator of type I interferon by mediating 'Lys-48'-linked ubiquitination of IRF3 and IRF7, leading to their degradation by the proteasome. Catalyzes ubiquitination and degradation of CAND2.
Protein Name Ubiquitin-Protein Ligase E3c
Hect-Type Ubiquitin Transferase E3c
Homologous To E6ap Carboxyl Terminus Homologous Protein 2
Hecth2
Rta-Associated Ubiquitin Ligase
Raul
Database Links Reactome: R-HSA-983168
Alternative Antibody Names Anti-Ubiquitin-Protein Ligase E3c antibody
Anti-Hect-Type Ubiquitin Transferase E3c antibody
Anti-Homologous To E6ap Carboxyl Terminus Homologous Protein 2 antibody
Anti-Hecth2 antibody
Anti-Rta-Associated Ubiquitin Ligase antibody
Anti-Raul antibody
Anti-UBE3C antibody
Anti-KIAA0010 antibody
Anti-KIAA10 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance