| Short Description : | Rabbit polyclonal anti-Ubiquitin-conjugating enzyme E2 N for use in WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | 1 mg/mL |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range: | WB 1:500-2000 |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | UBE2N |
| Gene ID: | 7334 |
| Uniprot ID: | UBE2N_HUMAN |
| Immunogen: | Synthesized peptide derived from human UBC13 |
| Specificity: | This antibody detects endogenous levels of UBC13 at Human, Mouse, Rat |
| Function | The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD. Promotes TRIM5 capsid-specific restriction activity and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate 'Lys-63'-linked polyubiquitin chains which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes. Together with RNF135 and UB2V1, catalyzes the viral RNA-dependent 'Lys-63'-linked polyubiquitination of RIGI to activate the downstream signaling pathway that leads to interferon beta production. UBE2V1-UBE2N together with TRAF3IP2 E3 ubiquitin ligase mediate 'Lys-63'-linked polyubiquitination of TRAF6, a component of IL17A-mediated signaling pathway. |
| Protein Name | Ubiquitin-Conjugating Enzyme E2 NBendless-Like Ubiquitin-Conjugating EnzymeE2 Ubiquitin-Conjugating Enzyme NUbc13Ubch13Ubiquitin Carrier Protein NUbiquitin-Protein Ligase N |
| Database Links | Reactome: R-HSA-1169408Reactome: R-HSA-168638Reactome: R-HSA-168927Reactome: R-HSA-202424Reactome: R-HSA-2871837Reactome: R-HSA-445989Reactome: R-HSA-450302Reactome: R-HSA-450321Reactome: R-HSA-5205685Reactome: R-HSA-5607764Reactome: R-HSA-5693565Reactome: R-HSA-5693571Reactome: R-HSA-5693607Reactome: R-HSA-5696395Reactome: R-HSA-69473Reactome: R-HSA-8866654Reactome: R-HSA-9020702Reactome: R-HSA-937039Reactome: R-HSA-937041Reactome: R-HSA-9646399Reactome: R-HSA-9705671Reactome: R-HSA-975110Reactome: R-HSA-975144Reactome: R-HSA-983168 |
| Cellular Localisation | NucleusCytoplasm |
| Alternative Antibody Names | Anti-Ubiquitin-Conjugating Enzyme E2 N antibodyAnti-Bendless-Like Ubiquitin-Conjugating Enzyme antibodyAnti-E2 Ubiquitin-Conjugating Enzyme N antibodyAnti-Ubc13 antibodyAnti-Ubch13 antibodyAnti-Ubiquitin Carrier Protein N antibodyAnti-Ubiquitin-Protein Ligase N antibodyAnti-UBE2N antibodyAnti-BLU antibody |
Information sourced from Uniprot.org
![Anti-Ube2N/Ubc13 antibody [SM5128] (STJA0005128)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/180181/403618/STJA0005128_1__27833.1738950626.png?c=1 "Anti-Ube2N/Ubc13 antibody [SM5128] (STJA0005128)")
