• STJ70299 staining (0. 5µg/ml) of HeLa lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
  • STJ70299 (0. 01µg/ml) staining of Human (A) , Mouse (B) , Rat (C) and Pig (D) Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • STJ70299 (0. 03µg/ml) staining of Human (A) , Mouse (B) and Rat (C) Skeletal Muscle lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • STJ70299 (0. 01µg/ml) staining of HEK293 (A) , HepG2 (B) and Jurkat (C) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • Immunofluorescence staining of MCF7 (A) and HeLa (B) cells with 5ug/ml STJ70299 antibody. Detected with Rabbit anti-goat IgG-Alexafluor488 antibody at 1:1000. Nuclei Counterstained with DAPI.

Anti-UBE2L3 antibody (C-Term) (STJ70299)

SKU:
STJ70299-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IF
Reactivity: Human/Mouse/Rat/Pig/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-UBE2L3 (C-Term) is suitable for use in ELISA, Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IF-Strong expression of the protein seen in the cytoplasm. Dividing cells appear to show enhanced expression. 5µg/ml
ELISA-antibody detection limit dilution 1:128000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: UBE2L3
Gene ID: 7332
Uniprot ID: UB2L3_HUMAN
Immunogen Region: C-Term
Accession Number: NP_003338.1
Immunogen Sequence: EFTKKYGEKRPVD
Post Translational Modifications Ubiquitinated. The alteration of UBE2L3 protein levels during the S-phase of the cell cycle is due to ubiquitin-dependent proteasomal degradation. Autoubiquitinated in vitro.
Function Ubiquitin-conjugating enzyme E2 that specifically acts with HECT-type and RBR family E3 ubiquitin-protein ligases. Does not function with most RING-containing E3 ubiquitin-protein ligases because it lacks intrinsic E3-independent reactivity with lysine: in contrast, it has activity with the RBR family E3 enzymes, such as PRKN, RNF31 and ARIH1, that function like RING-HECT hybrids. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Involved in the selective degradation of short-lived and abnormal proteins. Down-regulated during the S-phase it is involved in progression through the cell cycle. Regulates nuclear hormone receptors transcriptional activity. May play a role in myelopoiesis.
Protein Name Ubiquitin-Conjugating Enzyme E2 L3
E2 Ubiquitin-Conjugating Enzyme L3
L-Ubc
Ubch7
Ubiquitin Carrier Protein L3
Ubiquitin-Conjugating Enzyme E2-F1
Ubiquitin-Protein Ligase L3
Database Links Reactome: R-HSA-5357905
Reactome: R-HSA-5675482
Reactome: R-HSA-8866652
Reactome: R-HSA-8866654
Reactome: R-HSA-983168
Cellular Localisation Nucleus
Cytoplasm
Alternative Antibody Names Anti-Ubiquitin-Conjugating Enzyme E2 L3 antibody
Anti-E2 Ubiquitin-Conjugating Enzyme L3 antibody
Anti-L-Ubc antibody
Anti-Ubch7 antibody
Anti-Ubiquitin Carrier Protein L3 antibody
Anti-Ubiquitin-Conjugating Enzyme E2-F1 antibody
Anti-Ubiquitin-Protein Ligase L3 antibody
Anti-UBE2L3 antibody
Anti-UBCE7 antibody
Anti-UBCH7 antibody

Information sourced from Uniprot.org

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