Western blot analysis of extracts of various cell lines, using UBE2H antibody (STJ29483) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-UBE2H antibody (1-183) (STJ29483)

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STJ29483
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: UBE2H
Gene ID: 7328
Uniprot ID: UBE2H_HUMAN
Immunogen Region: 1-183
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-183 of human UBE2H (NP_003335.1).
Immunogen Sequence: MSSPSPGKRRMDTDVVKLIE SKHEVTILGGLNEFVVKFYG PQGTPYEGGVWKVRVDLPDK YPFKSPSIGFMNKIFHPNID EASGTVCLDVINQTWTALYD LTNIFESFLPQLLAYPNPID PLNGDAAAMYLHRPEEYKQK IKEYIQKYATEEALKEQEEG TGDSSSESSMSDFSEDEAQD MEL
Post Translational Modifications Autoubiquitinated in vitro in the presence of NEDD4L.
Function Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. E2 ubiquitin conjugating enzyme that transfers ubiquitin to MAEA, a core component of the CTLH E3 ubiquitin-protein ligase complex. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Capable, in vitro, to ubiquitinate histone H2A.
Protein Name Ubiquitin-Conjugating Enzyme E2 H
(E3-Independent E2 Ubiquitin-Conjugating Enzyme H
E2 Ubiquitin-Conjugating Enzyme H
Ubch2
Ubiquitin Carrier Protein H
Ubiquitin-Conjugating Enzyme E2-20k
Ubiquitin-Protein Ligase H
Database Links Reactome: R-HSA-8866652
Reactome: R-HSA-983168
Alternative Antibody Names Anti-Ubiquitin-Conjugating Enzyme E2 H antibody
Anti-(E3-Independent E2 Ubiquitin-Conjugating Enzyme H antibody
Anti-E2 Ubiquitin-Conjugating Enzyme H antibody
Anti-Ubch2 antibody
Anti-Ubiquitin Carrier Protein H antibody
Anti-Ubiquitin-Conjugating Enzyme E2-20k antibody
Anti-Ubiquitin-Protein Ligase H antibody
Anti-UBE2H antibody

Information sourced from Uniprot.org

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