| Applications: | WB/IF/ICC/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | UBE2G2 |
| Gene ID: | 7327 |
| Uniprot ID: | UB2G2_HUMAN |
| Immunogen Region: | 1-165 |
| Immunogen Sequence: | MAGTALKRLMAEYKQLTLNP PEGIVAGPMNEENFFEWEAL IMGPEDTCFEFGVFPAILSF PLDYPLSPPKMRFTCEMFHP NIYPDGRVCISILHAPGDDP MGYESSAERWSPVQSVEKIL LSVVSMLAEPNDESGANVDA SKMWRDDREQFYKIAKQIVQ KSLGL |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-165 of human UBE2G2 (NP_003334.2). |
| Function | Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Involved in endoplasmic reticulum-associated degradation (ERAD). Required for sterol-induced ubiquitination of 3-hydroxy-3-methylglutaryl coenzyme A reductase and its subsequent proteasomal degradation. |
| Protein Name | Ubiquitin-Conjugating Enzyme E2 G2E2 Ubiquitin-Conjugating Enzyme G2Ubiquitin Carrier Protein G2Ubiquitin-Protein Ligase G2 |
| Database Links | Reactome: R-HSA-8866652Reactome: R-HSA-983168 |
| Cellular Localisation | Endoplasmic ReticulumLipid Droplet |
| Alternative Antibody Names | Anti-Ubiquitin-Conjugating Enzyme E2 G2 antibodyAnti-E2 Ubiquitin-Conjugating Enzyme G2 antibodyAnti-Ubiquitin Carrier Protein G2 antibodyAnti-Ubiquitin-Protein Ligase G2 antibodyAnti-UBE2G2 antibodyAnti-UBC7 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and ISG15 Rabbit polyclonal antibody knockdown (KD) HeLa cells, using [KD Validated] ISG15 Rabbit polyclonal antibody (STJ11104641) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/177285/368924/STJ11104641_1__76093.1713134403.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and ISG15 Rabbit polyclonal antibody knockdown (KD) HeLa cells, using [KD Validated] ISG15 Rabbit polyclonal antibody (STJ11104641) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
